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Life Sciences

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Published yearly: 

4 Issues

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ISSN: 2320-964X (Online) 

ISSN: 2320-7817  (Print)

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Editor-in-Chief

Dr. Santosh Pawar 


INDEXED IN

ORIGINAL ARTICLE

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Int. Journal of Life Sciences, 2016; 4(1):137-139 |  Available online, April 11, 2016

Studies of water borne E.coli from the Congo red medium differentiation between pathogenic & non pathogenic in the broiler farms in Madhya Pradesh

 

Mishra Priti1, Ther Sandip V2 , Shukla Satish2*

1College of Fishery Science, Nanaji  Deshmukh Veterinary Science University Jabalpur, (M.P. India              

2Central Poultry Diagnostic  Laboratory (Phoenix Group) Jabalpur (M.P.) India

*Corresponding author E-mail : skshukla24@gmail.com 

Received: 25.01.2016   |   Revised : 05.03.2016   |   Accepted: 16.03.2016   |  Published : 11.04.2016

To aim of this study was to isolate and to identify the pathogenicity  of  E. coli from the water in poultry farms.  To conduct this study, water samples were collected from the poultry farms in the vicinity of Jabalpur. Around 200 water samples were collected from the 60 poultry farms, during the period of September 2011 to January 2014. All the water samples were cultured on Mac-Conkey’s and EMB agar plates. The positive samples were examined for coliform. confirmation of E. coli was based on the morphology of colonies, gram staining and biochemical characters. In vitro pathogenicity test for E. coli was carried out through the Congo-red binding activity. Out of 200 water samples processed for coliforms, 150 produced growth on Mac-Conkey’s agar. Biochemical characteristics identified 66 of these as E. coli. Out of 66 isolates of E. coli, 40 resulted in the growth of brick red colonies shown the pathogenic. While remaining 26 samples produced colorless colonies after 72 hours of incubation at room temperature and were confirmed as non pathogenic.

 

Keywords: Water, E. coli, Congo Red, Broilers poultry farms, Madhya Pradesh.

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Editor: Dr. Arvind Chavhan

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Cite this article as:

Mishra Priti, Ther Sandip V, Shukla Satish (2016) Studies of water borne E.coli from the Congo red medium differentiation between pathogenic & non pathogenic in the broiler farms in Madhya Pradesh. International J. of Life Sciences, 4(1): 137-139.

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Copyright: © 2016 | Author(s), This is an open access article under the terms of the Creative Commons Attribution-Non-Commercial - No Derivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.


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    INTERNATIONAL JOURNAL OF

    LIFE SCIENCES

    Print ISSN : 2320-7817 

    Online ISSN:2320-964X

    UGC Approved Journal No. 48951


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