Image description
Image description

Published yearly: 

4 Issues


ISSN: 2320-964X (Online) 

ISSN: 2320-7817  (Print)



Dr. Santosh Pawar 




Int. Journal of Life Sciences, 2016; 4(2):176-184 |  Available online,July 23, 2016

Microsatellite DNA marker based genetic polymorphism

in Wallago attu


Muhammad Muddassar Zafar1, Khalid Abbas1, Hafsa Basharat2*, Tanveer Ahmad1 and Uzma Hussain1


1Aquaculture Biotechnology Laboratory, Department of Zoology, Wildlife and Fisheries, University of Agriculture, Faisalabad-38040, Pakistan;

2Department of Zoology, University of Sargodha (Faisalabad Campus), Faisalabad-38040, Pakistan

*Correspondence author’s e-mail:


Received: 05.05.2016   |    Accepted: 16.06.2016   |   Published : 23.07.2016

Genetic diversity and structure of six Populations of Wallago attu were studied from Jhelum River, Pakistan using five species specific microsatellites loci. Genotypic data of all the samples were generated through PCR amplification of targeted loci. A total of 23 alleles were found in 177 fish samples. All the microsatellite loci were found to be polymorphic. The mean FST value (0.0248) showed little genetic differentiation. The average observed heterozygosity values were measured in range from 0.3450 to 0.4400 whereas average expected heterozygosity ranging from 0.5363 to 0.5777 were recorded in all the population. Majority of examined loci significantly deviated (P<0.05) from Hardy Weinberg Equilibrium (HWE). Analysis of Molecular Variance (AMOVA) indicated that majority of the variation lies within the individuals (81.10%) than among the individuals with in populations (16.4%).The results showed genetic decline in this valuable fish resource. The findings of the present study will be helpful for management, conservation as well as least kinship selective breeding programs for W. attu.

Key words: Genetic Variation, Freshwater Shark, Population Structure, Molecular Marker. 



Editor: Dr. Arvind Chavhan


Cite this article as:

Muhammad Muddassar Zafar, Khalid Abbas, Hafsa Basharat, Tanveer Ahmad and Uzma Hussain (2016) Microsatellite DNA marker based genetic polymorphism in Wallago attu, International J. of Life Sciences, 4 (2): 176-184.



The authors express their sincere thanks to Irum Qadeer and Nadia Nazish doctorate scholars, Aquaculture Biotechnology Lab. Department of Zoology, Wildlife and Fisheries for their scholarly guidance and constant encouragement during the course of research work.



Copyright: © 2016 | Author(s), This is an open access article under the terms of the Creative Commons Attribution-Non-Commercial - No Derivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.







    Print ISSN : 2320-7817 

    Online ISSN:2320-964X

    UGC Approved Journal No. 48951


    46, Guruwandan, Jawahar Nagar, 

    VMV Road, Amravati- 444604

    Maharashtra, India.

    Tel  + 91- 9970559438