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Published yearly: 

4 Issues


ISSN: 2320-964X (Online) 

ISSN: 2320-7817  (Print)



Dr. Santosh Pawar 




Int. Journal of Life Sciences, 2016; 4(3):373- 378   |    Available online, October 8, 2016

Screening and characterization of bioethanol producing yeasts from various sources


Sundarrajan Priya*, Shetty Sangeeta and Shigvan  Aniket


Department of Life Science and Biochemistry, St. Xavier’s College (Autonomous), 5, Mahapalika Marg,

Mumbai, India - 400001.

*Corresponding Author, email:


Received: 24.05.2016   |   Accepted: 13.08.2016   |   Published : 08.10.2016

The use of bioethanol as an alternative, renewable and green source of fuel has been steadily increasing around the world. Ethanol can be made synthetically from petroleum or by microbial conversion of biomass through fermentation. In the present study, nine ethanol-producing yeasts were isolated from various sources like soil, sugarcane bagasse and rotten fleshy fruits. Morphological, microscopic and biochemical studies were carried out with these isolates. The yeasts were characterized for temperature tolerance, osmotolerance and growth with sugar sources at various pH. Ethanol production was estimated by Nitroblue tetrazolium assay. Different strains showed ethanol production with 2% of glucose, sucrose, galactose, lactose, maltose and fructose. One of the strains isolated showed high osmotolerance with growth up to 70% sugar. The strains grew at different pH ranging from 3.5 to 9 and temperature ranging from 20°C to 37°C. For one strain, maximum ethanol production was observed at 25°C, pH 5.5. This strain produced 43.9 mg/ml ethanol from 80 mg/ml of glucose after 72 hours of fermentation indicating that the strain has a potential for industrial application.


Keywords: Yeasts, Bioethanol, Fermentation, Nitroblue tetrazolium assay.



Editor: Dr. Arvind Chavhan


Cite this article as:

Sundarrajan Priya, Shetty Sangeeta and Shigvan  Aniket (2016) Screening and characterization of bioethanol producing yeasts from various sources, International J. of Life Sciences, 4 (3): 373-378.


Acknowledgement: The authors would like to thank the Principal, St. Xavier's College (Auto.), Mumbai for providing the space and infrastructure to carry out this research work. The authors would also like to thank Dr. Vishwas Sarangdhar, Consultant, Caius Research Laboratory, St. Xavier’s College (Autonomous), for providing technical advice for conducting the study and help in review of  manuscript.


Conflicts of interest: The authors stated that no conflicts of interest.


Copyright: © 2016 | Author(s), This is an open access article under the terms of the Creative Commons Attribution-Non-Commercial - No Derivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

Bai FW, Anderson WA and Moo-Young M (2008) Ethanol fermentation technologies from sugar and starch feedstocks. Biotechnol. Adv. 26: 89-105.

Gupta N, Dubey A and Tewari L (2009) High efficiency alcohol tolerant Saccharomyces isolates of Phoenix dactylifera for bioconversion of sugarcane juice into bioethanol, Journal of Scientific and Industrial Research. 68, 401-405.

Lin Y and Tanaka S  (2006) Ethanol fermentation from biomass resources: Current state and prospects. Appl. Microbiol. Biotechnol. 69: 627-642.

Basso LC, Basso TO and Rocha SA (2011) Ethanol Production in Brazil; The Industrial Process and Its Impact on Yeast Fermentation, Biofuel Production-Recent Developments and Prospects, Dr. Marco Aurelio Dos Santos Bernardes (Ed.), ISBN: 978-953-307-478-8.

Scheel M and Lutke-Eversloh T (2013) New options to engineer biofuel microbes: Development and application of a high-throughput screening system. Metabolic Engineering. 17, 51-58.

Ali  MN and Khan  MM (2014) Screening, identification and characterization of alcohol tolerant potential bioethanol producing yeasts. Current Research in Microbiology and Biotechnology. 2, 316-324.

Osho A (2005) Ethanol and sugar tolerance of wine yeasts isolated from fermenting cashew apple juice. African Journal of Biotechnology. 4, 660-662.

Patil  SK and Patil AB (2006) Isolation and characterization of wine yeast from pineapple fruits. Karnataka Journal of Agricultural Sciences. 19, 558-561.






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