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ISSN: 2320-964X (Online) 

ISSN: 2320-7817  (Print)



Dr. Santosh Pawar 




Int. Journal of Life Sciences, 2016; 4(3):385- 393   |    Available online, October 25, 2016

Microsatellite markers revealed poor genetic structure of Wallago attu in Punjab, Pakistan



Hafsa Basharat1, Abdul Ghafoor1, Arvind Chavhan3* Muhammad Muddassar Zafar2,*Khalid Abbas2, Javeria Tabasum1and Razia Parveen1



1Department of Zoology, University of Sargodha, Sargodha-40100, Pakistan  | 2Aquaculture Biotechnology Laboratory, Department of Zoology, Wildlife and Fisheries, University of Agriculture, Faisalabad-38040, Pakistan. |  3Department of Zoology, D.B. College, Bhokar, MS, India-431801

*Correspondence author’s e-mail:

Received: 05.07.2016    |    Accepted: 25.08.2016    |    Published : 25.10.2016

Understanding with genetic variation is important for management of wild populations and these variations are imperative for maintaining evolutionary potential and ensuring characteristics for wild populations. Genetic diversity and population structure of Wallago attu was assessed across its natural distribution in Punjab using five species specific microsatellite markers. A total of 177 fish samples was taken and genotypic data of all the samples were generated through PCR amplification of targeted loci. All microsatellite proved to be polymorphic, a total of 36 alleles were found in five sampled populations whose number of allele varied from 6 to 9.  The mean FST value 0.0269 revealed little genetic differentiations. The mean observed heterozygosity was ranged from 0.5328 (CB) to 0.8225 (MD) and the expected heterozygosities were found in range from 0.5396 (CB) to 0.8341 (MD). Significant deviations (p<0.05) from Hardy-Weinberg Equilibrium (HWE) have been observed in all five populations. The results purposed poor genetic structuring in this valuable resource. The knowledge generated and tools used in this research work will be helpful for the future commercial development of W. attu in terms of selective breeding and for better understanding of effective fisheries management.

Key Words: Allelic Polymorphism, Freshwater Shark, Population Structure, Molecular Marker.



Editor: Dr. Jadhao R.G. 


Cite this article as:

Hafsa Basharat, Abdul Ghafoor, Arvind Chavhan, Muhammad Muddassar Zafar,Khalid Abbas, Javeria Tabasum and Razia Parveen (2016) Microsatellite markers revealed poor genetic structure of Wallago attu in Punjab, Pakistan, International J. of Life Sciences, 4 (3): 385-393.



The authors express their sincere thanks to Mr. Tanveer Ahmad and Ms. Nadia Nazish doctorate scholars, Aquaculture Biotechnology Lab. Department of Zoology, Wildlife and Fisheries, University of Agriculture, Faisalabad for their scholarly guidance, moral support and constant encouragement during the course of research work.


Conflicts of interest: The authors stated that no conflicts of interest.


Copyright: © 2016 | Author(s), This is an open access article under the terms of the Creative Commons Attribution-Non-Commercial - No Derivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.



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