Image description

Life Sciences

Image description

Published yearly: 

4 Issues

______________________

ISSN: 2320-964X (Online) 

ISSN: 2320-7817  (Print)

______________________

Editor-in-Chief

Dr. Santosh Pawar 


INDEXED IN

ORIGINAL ARTICLE

___________________________________________________________________________________

Int. Journal of Life Sciences, 2016; 4(3):394- 400   |    Available online, October 8, 2016

Exploration of Alkaliphilic Actinomycetes for Amylase Production from Agroindustrial Waste

 

 

Vidhale NN and Pundkar VM

  


Department of Microbiology, Shri Shivaji science college, Amravati, 444603 (M.S.), India

 

Received: 05.07.2016    |   Accepted: 09.08.2016   |   Published : 25.10.2016

Saline soil possessing high pH value (above 8.0) predominates alkaliphilic actinomycetes (Singh S et al., 2010), which can be explored for the production of amylase from agroindustrial waste as substrate using solid state fermentation. In this study six types of agroindustrial waste such as wheat bran, rice bran, Dal mil waste, oil mil waste, vegetable waste and molasses were employed. Solid state fermentation (SSF) was carried out at various moisture content (10 to 35%) at 37˚ c. results indicates that alkaliphilic Actinomycetes from saline soil of vidarbha region produced maximum amylase in vegetable waste (4.45mg/ml) in presence of 30 % moisture after 96 hrs, followed by oil mil waste (4.5mg/ml) in presence of 25%moisture after 120hrs, wheat bran and rice bran (3.9mg/ml) at 25%moisture content after 120hrs and 96hrs respectively. Dal mil waste was found to produce 3.5mg/ml amylase in presence of 25% moisture after 96hrs and molasses showed very least production of amylase (2mg/ml) after 144hrs. Hence it indicates that agroindustrial wastes are good source for the production of amylase using alkaliphilic Actinomycetes.

 

Key words: Amylase, Actinomycetes, saline soil, Agroindustrial waste.

 

_________________________________________________________________________

Editor: Dr. Arvind Chavhan

___________________________________________________________________________________ 

Cite this article as:

Vidhale NN and Pundkar VM (2016) Exploration of Alkaliphilic Actinomycetes for Amylase Production from Agroindustrial Waste, International J. of Life Sciences, 4 (3): 394-400.

_________________________________________________________________________


Conflicts of interest: The authors stated that no conflicts of interest.

_________________________________________________________________________

Copyright: © 2016 | Author(s), This is an open access article under the terms of the Creative Commons Attribution-Non-Commercial - No Derivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

_________________________________________________________________________

 

Deshmukh AA, Vidhale NN (2014) Screening the antibacterial actinomycetes from saline soil of vidarbha region..I. J. App. Research.;4(3)20-21.


George L Peltier and Beckord LD (1945) Sources of amylase-producing Bacteria.. J. Bacteriology.; 50(6): 711-714.


Kuo MJ and Harman PA (1966) isolation of amylolytic strains of thermoactinomycetes vulgaris and production of thermophilic actinomycetes amylases.. J. bacteriology.; vol.92,no.3,723-726.


Karanwal Akansha and Varsha Nigam (2013) Production of Amylase enzyme by isolated microorganisms and its application.. Int. J. Pharm. and Bio. Sci.; 3(4): 354-360.


Maller A, Andre Ricaedo Lima Damasio, Tony Marcio da Silva, Joao Atilio Jorge, Hector Francisco Terenzi and 

Maria de Lourdes Teixeira de Moraes Polizeli (2011) Biotechnological Potential of Agro-industrial waste as carbon source to thermostable polygalacturonase production in Aspergillus niveus.. Enz. Res.article.ID 289206,; 10(4061):pg.,6.


Poornima R, Maloy Kumar Sahu K Sivakumar and  Pushpavalli V (2010) Optimization of α-amylase production by actinomycetes strain AE-19 isolated from shrimp pond. 2008. Acdemic journals Inc.Trends in applied sciences research.;3(1)45-52.


Renge VC, Khedkar SV and Nikita R Nandurkar. (2012) Enzyme synthesis by fermentation method.:A review. Sci. revs. Chem.. commun.;2(4),585 -590.


Singh SP, Thakur JT, Gohel SD, and Purohit MK (2010)  Molecular diversity and enzymatic potential of salt-tolerant  alkaliphilic  actinomycetes. Formatex .info; microbiology2 /280- 286.


Selvam K, Vishnupriya B and  Subhash Chandra Bose V (2011) Screening and Quantification marine actinomycetes producing industrial enzymes Amylase, Cellulose and Lipase from south coast of India.. Int. J. Pharmaceutical and biological archives.; 2(5):1481-1487.


Suganthi R, Benazir JF, Santhi R, Rameshkumar V, Anjana Hari, Nitya Meenakshi, Nidhiya KA, Kavitha G, 

Lakshmi R (2011) Amylase production by aspergillus niger under solid state fermentation using agroindustrial wastes.. Int. J. Engineering sci. and tech.;3(2):1756-1763.


Vidyalakshmi R, Paranthaman R and Indhumati J (2009) Amylase production on Submerged fermentation by Bacillus spp.. World J. chemistry; 4(1): 89-91.

IJLSCI EVENTS

National Conference 

COMING SOON


ADVERTISEMENT

 
















    INTERNATIONAL JOURNAL OF

    LIFE SCIENCES

    Print ISSN : 2320-7817 

    Online ISSN:2320-964X

    UGC Approved Journal No. 48951


    EDITORIAL OFFICE

    46, Guruwandan, Jawahar Nagar, 

    VMV Road, Amravati- 444604

    Maharashtra, India.

    Tel  + 91- 9970559438

    Email: editor@ijlsci.in