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Life Sciences

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Published yearly: 

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ISSN: 2320-964X (Online) 

ISSN: 2320-7817  (Print)

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ORIGINAL ARTICLE

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Int. Journal of Life Sciences, 2017; 5(1):46-54  |    Available online, 31 March, 2017

Transcript profiling of vital defense proteins upregulated during 3, 5-Dichloroanthranilic acid (DCA) mediated induced resistance against pearl millet downy mildew disease

 


Lavanya SN1, Niranjan-Raj S2 and Amruthesh KN1

1Department of Studies in Botany, University of Mysore, Manasagangotri, Mysore-570006, India.

2Department of Studies in Microbiology, Karnataka State Open University, Mukthagangotri, Mysore-570006, India.

*Corresponding authors:  Dr. Amruthesh KN  | Email: niruraj@gmail.com

Received: 10.12.2016 | Accepted: 29.01.2017 | Published : 31.03.2017

Downy mildew disease caused by the biotrophic oomycete Sclerospora graminicola is the main constraint for pearl millet production incurring huge yield and economic losses. The synthetic resistance elicitor 3, 5-Dichloroanthranilic acid (DCA) when applied exogenously as seed treatment to pearl millet at the concentration of 100 μM protected pearl millet plants by inducing systemic and durable resistance against downy mildew. This enhanced resistance correlated with the up regulation of various defense genes and proteins. Transcripts of mRNA of the defense enzymes PAL and POX were significantly enhanced many folds in comparison to the untreated control. The cell wall cross-linking protein HRGPs also showed significant overexpression in DCA treated seedlings compare to the control. Pathogenesis related proteins PR-1 and PR-5 which are regarded as markers of induced resistance were significantly over expressed in DCA treated seedlings, and expression of PR-5 was more than that of the resistant check. Early and increased expression of defense enzymes PAL and POX and defense proteins HRGPs, PR-1 and PR-5 are attributed as causes for enhanced DM protection that is offered by DCA treatment to pearl millet seeds. Changes in transcriptional profiles of defense enzyme sand proteins triggered by DCA clearly resemble typical defense-related responses elicited during elicitor induced resistance suggesting a potential for DCA in management of plant disease in general and pearl millet downy mildew disease in particular.

 

Keywords: Pearl millet downy mildew, induced resistance, defense genes, PR-proteins.

 

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Editor: Dr. Arvind Chavhan

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Cite this article as:

Lavanya SN, Niranjan-Raj S and Amruthesh KN (2017) Transcript profiling of vital defense proteins upregulated during 3, 5-Dichloroanthranilic acid (DCA) mediated induced resistance against pearl millet downy mildew disease, International J. of Life Sciences, 5 (1): 46-54.

 

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Acknowledgement:

The authors are grateful to University Grands Commission (UGC) for financial assistance under Major Research Project sanctioned to corresponding author. The facilities provided by the Indian Council of Agricultural Research (ICAR), the Government of India, through the All-India Coordinated Research Project on Pearl Millet Improvement Program (AICRP-PM) is also gratefully acknowledged.

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Conflicts of interest: The authors stated that no conflicts of interest.

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Copyright: © 2017 | Author(s), This is an open access article under the terms of the Creative Commons Attribution-Non-Commercial - No Derivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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