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Published yearly: 

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ISSN: 2320-964X (Online) 

ISSN: 2320-7817  (Print)



Dr. Santosh Pawar 




Int. Journal of Life Sciences, 2017; 5(2): 227-232             |        Available online, June 30, 2017

Effect of copper sulphate on the regulation of nitrogen metabolism in the Rita rita fish

Banerjee Suchitra1, Vyas Rashmi2 and Anita Tomar2


1Govt. Exellance College, Bhopal (M.P.)

2M.L.B Girls P.G. Auto. College, Bhopal (M.P.)

Received: 13.01.2017    |     Accepted: 04.05.2017    |      Published :30.06.2017

Effect of copper sulphate (CuSO4.5H2O) to Rita rita (catfish) species was investigated using toxicity index of 96 hours LC50 and the quantal response determined by the statistical probit analysis method. In response to the lethality of the copper toxicant, behavioral anomalies (locomotor response) of the exposed fish species were studied as indication of toxic effects of the heavy metal. Fish species shows different mortality responses to the varying concentrations of copper sulphate studied (10 ppm to 50 ppm) due to toxicity. Copper was significantly (no overlap in 95% C.L of 96 hrs LC50 values) more toxic to Rita rita fish. 96 hrs LC50 values were revealed to be 34 mg/l. On termination of the experimental period (7 days, 15 days and 30 days) the biochemical analysis: tissue protein; total free amino acids; ammonia; urea and glutamic acid. There is need to control the use of copper because of its observed toxicity and fish avoidance test shows to be an important predictive and sensitive biomarker in aquatic monitoring and pollution management.


Key words: Copper sulphate, Rita rita, 96 hrs LC50



Editor: Dr.Arvind Chavhan


Cite this article as:

Banerjee Suchitra, Vyas Rashmi and Anita Tomar (2017) Effect of copper sulphate on the regulation of nitrogen metabolism in the Rita rita fish; International J. of Life Sciences, 5  (2): 227-232.


Conflicts of interest: The authors stated that no conflicts of interest.



Copyright: © 2017 | Author(s), This is an open access article under the terms of the Creative Commons Attribution-Non-Commercial - No Derivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.


Bayne BL, Brown DA, Burns K, Dixon DR, Vanovici AL and Livingstone DR (1985) The effects of stress and pollution in marine animals Praeger Publishers, New York, p. 235-248.

Bayne BL, Clarke KR and Moore MN (1981) Some practical considerations in the measurement of pollution effects on bivalve mollusks, and some possible ecological consequences Aquat. Toxicol., 1: 159-174.

Bergmeyer HU (1965), Methods of Enzymatic analysis, Academic Press, New York, 401 pp.

Dange AD, Masurekar VB (1985) Toxicity of toluene: effect of aminotransferases in different tissues of Tilapia mossambica (Peters) adapted to seawater Symp. Ser. Mar. Biol. Ass. India, 6 : 833-838.

Doudoroff P (1953) Some recent developments in the study of toxic industries wastes. Proc. 4th Pacific north-west industr. Waste Conf. State Coll. Washington, Pullman, p. 21-25.

Lopes PA, Pinheiro T, Santos MC, Mathias M, Collares-Pereira MJ, Viegas-Crespo AM (2001) Response of ant ioxi dant  enzymes i n fr eshwater  fi sh populations (Leuciscus alburnoides complex) to inorganic pollutants exposure. Sci Total Environ; 280: 153-163.

Lopes PA, Pinheiro T, Santos MC, Mathias M, Collares-Pereira MJ, Viegas-Crespo AM. Response of antioxidant enzymes in freshwater fish populations (Leuciscus alburnoides complex) to inorganic pollutants exposure. Sci Total Environ. 2001; 280: 153-163.

Lowry OH, Rosenbrough NJ, Farr AJ and Randall RJ (1951) Protein measurement with Folin-Phenol reagent. J. Biol. Chem., 193 : 265-275.

Mikhaylova LV (1983) Complex formation between the water- soluble petroleum fraction and blood and liver proteins of carp Cyprinus carpio. J. Ichthyol., 23 (3): 154-158.

Moore S and Stein WH (1954) In: Colowick and Kaplan. (Eds.) Methods in Enzymology, Vol II, Academic Press, New York.

Natelson S (1971) Techniques of clinical chemistry. Thomas CC Publishers, Springfield, Illinois, 146 : 725.

Robert K Murray, Daryl K Granner, Peter A Mayes, Victor W Rodwell (1998) Harper’s Biochemistry, Lange Medical Publications, Maruzen, Asia.

Rudolph A, Yanez R, Troncoso L Gonazalez (2002) Stimulation of enzymatic defense mechanisms and appearance of liver damage in juvenile trout, Oncorhynchus mykiss exposed to water-accommodated trace petroleum residues. Bull. Environ. Contam. Toxicol., 68: 644-651.

Stickle WB, Rice SD  and Moles A (1984) Bioenergetics and survival of the marine snail Thais lima during long-term oil exposure. Mar. Biol., 80:281.

Tucker CS, Robinson EH. Channel Catfish Farming Handbook. Van-Nostrand-Reinhold, New York, USA; 1990.

Van der Oost R, Beyar J, Vermeulen NPE (2003) Fish bioaccumulation and biomarkers in environmental risk assessment: A review. Environmental Toxicology and Pharmacology; 13: 57-149.

Wekell MMB and Brown GW (1973) Ornithine aminotransferase of fishes. Comp. Biochem. Physiol., 46:779-795.

Wu RSS, Lam KS, MacKay DW, Lau TC and Yam V (1994) Impact of marine fish farming on water quality and bottom sediment: A case study in the subtropical environment. Mar. Env. Res. 38: 115-145.

Wur t s WA, Per schbacher PW (1994)  Effects of bicarbonate alkalinity and calcium on the acute toxicity of copper to juvenile channel catfish (Ictalurus punctatus). Aquaculture, 125: 73– 79.1.







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