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Life Sciences

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Published yearly: 

4 Issues

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ISSN: 2320-964X (Online) 

ISSN: 2320-7817  (Print)

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ORIGINAL ARTICLE

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Int. Journal of Life Sciences, 2017; 5(2): 277-282             |        Available online, July 07, 2017

Isolation and screening of cellulolytic bacteria from soil and optimization of cellulase production


Shilpa Lokhande and Pethe AS

 

 

Department of Microbiology, Shri Shivaji College of Arts, Commerce and Science, Akola (M.S.), India – 444003

Email : shilpa.lokhande77@gmail.com  |  archanapethe14@gmail.com

 

Received: 02.08.2016    |     Accepted: 25.06.2017    |      Published :07.07.2017

The present investigation was undertaken to isolate and Screen the Cellulase Producing bacteria. Microbial cultures were isolated from the soil sample collected from different villages of saline belt of Akola and Buldhana District, Maharashtra India. A Total of 146 isolates were isolated  and identified based on Morphology and Biochemical characterization. Among all isolated organisms 37 bacterial species were isolated, the one cellulolytic bacterial isolate shows maximum enzyme activity, and identified as Bacillus thuringiensis. The best conditions like pH, carbon source, temperature and incubation period were also observed for cellulase producing organisms. Carboxy methyl cellulose [1.0% (w/v)] were found to be the best  carbon  source for the production of cellulase and  Optimum temperature and pH of the medium for the cellulase were 40°C and 7, whereas it shows maximum enzyme activity after third day of incubation period.

 

Keyword : Cellulose, cellulase, Bacillus thuringiensis


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Editor: Dr.Arvind Chavhan

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Cite this article as:

Shilpa Lokhande and Pethe AS  (2017) Isolation and screening of cellulolytic bacteria from soil and optimization of cellulase production; International J. of Life Sciences, 5  (2): 277-282. 

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Conflicts of interest: The authors stated that no conflicts of interest.

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Copyright: © 2017 | Author(s), This is an open access article under the terms of the Creative Commons Attribution-Non-Commercial - No Derivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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