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Published yearly: 

4 Issues


ISSN: 2320-964X (Online) 

ISSN: 2320-7817  (Print)



Dr. Santosh Pawar 




Int. Journal of Life Sciences, 2017; 5(2): 211-218             |        Available online, June 30, 2017

Micropropagation of Plumbago zeylanica L. using in vitro germinated seedling explants

Kakade Prachi Sharad and Malpathak Nutan P*


Department of Botany, Savitribai Phule Pune University, Pune – 411007 (MH.) India.

*, office: +91-020-25601438, 25601439, fax: +91-020-25690498


Received: 03.05.2017    |     Accepted: 15.06.2017    |      Published :30.06.2017

In vitro seed germination in Plumbago zeylanica L. has been achieved using combined treatment of seed storage, seed soaking, mechanical scarification and MS-basal medium supplemented with GA3. P. zeylanica seeds stored for six months soaked in distilled water for 30h showed maximum seed germination (95.55%) on ½–Murashige and Skoog basal+GA3 (3.61 µM). Hardening and survival of seedlings obtained from seed germination was 86%. Maximum multiple shoots/explant (18.02±0.09) proliferated from nodal explants cultured on MS-basal medium supplemented with BAP, Kn and Zea (4.44+4.56+2.32 µM). Maximum shoots rooted on ½–MS basal+IBA (6.15 µM) having 21.91±1.44 roots/shoot and root length 9.36±0.56 cm. Successful hardening of plantlets showed 94% of survival after transferring to garden condition. To the best of our knowledge, this is the first report where in vitro developed seedling has been used as an explant source which will nullify the level of contamination in cultures. We have successfully established an efficient protocol for in vitro seed germination, multiple shoot production through nodal culture and rooting of regenerated shoots to achieve an optimized plant regeneration system along with acclimatization in natural condition. This protocol can be used for large scale commercial micropropagation and conservation purpose.


Keywords - Plumbagin, seed storage, mechanical scarification, seed germination, multiple shoot induction.


Editor: Dr. Arvind Chavhan


Cite this article as:

Kakade Prachi Sharad and Malpathak Nutan P (2017) Micropropagation of Plumbago zeylanica L. using in vitro germinated seedling explants; International J. of Life Sciences, 5  (2): 211-218.



The authors are thankful to the Department of Science and Technology, Ministry of Science and Technology, Government of India for the research grant under Women Scientist Scheme (WOS – A) (SR/WOS-A/LS-367/2012).


Conflicts of interest: The authors stated that no conflicts of interest.



Copyright: © 2017 | Author(s), This is an open access article under the terms of the Creative Commons Attribution-Non-Commercial - No Derivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.


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