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Published yearly: 

4 Issues

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ISSN: 2320-964X (Online) 

ISSN: 2320-7817  (Print)

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Editor-in-Chief

Dr. Santosh Pawar 


INDEXED IN

ORIGINAL ARTICLE

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Int. Journal of Life Sciences, 2017; 5(4): 491-498             |        Available online, November 21, 2017

Molecular characterization of Infectious Bursal Disease virus from gamefowls in selected areas in CALABARZON, Philippines


Caceres Abbegaile B  DVM1, Umali Dennis V DVM, PhD1

Bernardo Francis Andrew Eugene M. DVM, MSand Katoh Hiromitsu DVM, PhD1,2

 


1Department of Veterinary Clinical Sciences, College of Veterinary Medicine, University of the Philippines, Los Baños, Laguna, 4031, Philippines

2Poultry Products Quality Control (PPQC), 125-7 Daiwa Dakeonsen, Nihonmatsu, Fukushima, 964-0062, Japan

Correspondence to: Dennis V. Umali, DVM, PhD  |  Email: dvumali@up.edu.ph  |  Contact : +63 49 536 2730

 

Received : 15.10.2017  |   Accepted : 09.11.2017    |   Published : 21.11.2017

Infectious Bursal Disease (IBD) is a highly infectious, economically important, immunosuppressive viral disease of poultry. In this study, four field strains of IBD virus (IBDV) from gamefowls in selected areas in CALABARZON, Philippines were characterized. Clinical signs observed in affected chickens were inappetence, white scours, ruffled feathers, lethargy and acute increased in mortalities. Gross lesions observed were enlarged hemorrhagic cream colored bursae with yellowish exudates. Hemorrhages in the junction of the proventriculus and gizzard were also noted. Nucleotide sequencing showed that the field strains belong to serotype-1 with deduced amino acid sequences of 222A, 256I, 294I, 242I and 299S at the VP-2 gene, which indicates that the field strains were from the very virulent IBDV (vvIBDV) genotype. Phylogenetic analysis showed that the field strains belong to the European-like vvIBDV subgenotype and were closely related to (97-98%) to Spanish, South African and Nigerian IBDV strains. At present, this is the first molecular characterization of IBDVs in the Philippines.

 

Key words: Calabarzon, gamefowl, Infectious bursal disease, phylogenetic analysis, vvIBD.


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Editor: Dr.Arvind Chavhan

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Cite this article as:

Caceres Abbegaile B, Umali Dennis V, Bernardo Francis Andrew Eugene M and Katoh Hiromitsu (2017) Molecular characterization of Infectious Bursal Disease virus from gamefowls in selected areas in CALABARZON, Philippines; International J. of Life Sciences, 5 (4): 491-498.

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Acknowledgement

The authors would like to thank Masako Katoh and PPQC Foundation for the technical and logistical support in the conduct of this study.

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Conflicts of interest: The authors stated that no conflicts of interest.

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Copyright: © 2017 | Author(s), This is an open access article under the terms of the Creative Commons Attribution-Non-Commercial - No Derivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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    INTERNATIONAL JOURNAL OF

    LIFE SCIENCES

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