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Published yearly: 

4 Issues

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ISSN: 2320-964X (Online) 

ISSN: 2320-7817  (Print)

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Editor-in-Chief

Dr. Santosh Pawar 


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ORIGINAL ARTICLE

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Int. Journal of Life Sciences, 2017; 5(4): 517-526             |        Available online, November 21, 2017

DNA Barcoding of Six Species of Family Rhopalidae (Insecta: Hemiptera: Heteroptera) from India


Kaur Harbhajan* and Sekhon Navpreet Kaur

 

 

Department of Zoology and Environmental Sciences, Punjabi University, Patiala.

*E-mail: harbhajankaur@hotmail.com

 

Received: 01.08.2017   |   Accepted: 09.11.2017    |      Published : 21.11.2017

Rhopalidae is a small family comprising 18 genera and 209 recognised species all over the world. The Oriental Realm embraces approximately 30 species of about 10 genera. In this study, first ever comprehensive DNA barcode analysis of 17 specimens of six species belonging to five genera of family Rhopalidae from northern parts of India has been presented and compared with worldwide available data. Sequences for all the species but Leptocoris augur (Fabricius, 1974) are first submissions from India while six sequences of Liorhyssus rubicundus (Signoret, 1859) are the pioneer submissions for this particular species to the GenBank and BOLD.

 

Both the species of genus Liorhyssus  viz L. hyalinus (Fabricius, 1794)  and L. rubicundus (Signoret, 1859) show striking phenotypic plasticity but can be easily delineated by distinct barcodes showing minimum interspecific K2P distance of 7.6 % and much lower maximum intraspecific K2P distances of  2.1% and 0.5% respectively. Our two sequences of Stictopleurus pictus (Fieber, 1861) show K2P distance of 0.0% but 2.3% with sequences from Germany. The Rhopalus (Aeschyntelus) sp. nr. maculatus shows average K2P distance of 3.2%  with Rhopalus maculatus (Fieber,1837) and appears to be a cryptic species. Corizus sp. shows distinct barcode which delineates it from its nearest neighbour Corizus hyocyami (Linnaeus, 1758).

 

Keywords: COI gene, Coreoidea, K2P distance, Evolutionary affinities

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Editor: Dr.R.G. Jadhao

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Cite this article as:

Kaur Harbhajan and  Sekhon Navpreet Kaur (2017) DNA Barcoding of Six Species of Family Rhopalidae (Insecta: Hemiptera: Heteroptera) from India; International J. of Life Sciences, 5 (4): 517-526. 

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Acknowledgement

We thank the Department of Zoology and Environmental Sciences, Punjabi University, Patiala and Sophisticated Instrumentation Centre, Punjabi University, Patiala for providing all the lab facilities. The funding for the research was provided by University Grants Commission (UGC) under the Basic Scientific Research Scheme -2013 (BSR-2013). The help and assistance provided by Professor Emeritus C.A Viraktamath and his team Dr. Yeshwanth HM and Dr. Nirmala P of Department of Entomology, University of Agricultural Sciences, Bangalore for identification of bugs is highly appreciated

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Conflicts of interest: The authors stated that no conflicts of interest.

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Copyright: © 2017 | Author(s), This is an open access article under the terms of the Creative Commons Attribution-Non-Commercial - No Derivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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