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Published yearly: 

4 Issues

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ISSN: 2320-964X (Online) 

ISSN: 2320-7817  (Print)

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Editor-in-Chief

Dr. Santosh Pawar 


INDEXED IN

ORIGINAL ARTICLE

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Int. Journal of Life Sciences, 2017; 5(4): 551-560             |        Available online, December 05, 2017

Arsenic induces oxidative stress and apoptosis in human lung cancer cells

 

 

Kiran Kumar KM1*, Naveen Kumar M1, Rashmi Nagesh1, Rajeshwari H Patil1, Babu RL 2.,

S Chidananda Sharma1and Manjunath K1


1Department of Microbiology and Biotechnology, Bangalore University, Jnana Bharathi, Bengaluru- 560 056, Karnataka, India

2Department of Bioinformatics and Biotechnology, Akkamahadevi Women’s University, Jnana Shakthi Campus, Vijayapura, 586 108, Karnataka, India

*Corresponding author e-mail- kirankumar.krkm@gmail.com

 

Received: 06.10.2017   |    Accepted: 20.11.2017   |  Published : 05.12.2017

Arsenic is a toxic heavy metal and a well-known environmental contaminant. Often water, food, soil are found to be contaminated with arsenic and occupational exposure leads to harmful health issues. Hence, in the present study human lung epithelial A549 cells were used as in vitro model system. Treatment of A549 cells with arsenic for 48 h showed reduced viability with increased leakage of lactate dehydrogenase with loss of membrane damage. Arsenic significantly induces reactive oxygen species with depletion of free radical neutralizing antioxidant glutathione peroxidase and catalase enzymes. IC50 value of 2 µM arsenic concentration was used for mRNA expression studies. Further, pro-apoptotic Bax gene was induced and anti-apoptotic Bcl-2 was decreased. Arsenic stimulates dysfunction of cell cycle progression by downregulation of Cyclin-E1and Cyclin-D1 mRNA expressions. Thus confirms the toxic nature of arsenic and clues to oxidative redox potential alteration with cell cycle inhibition and apoptosis in lung epithelial cells.

 

Key words: Arsenic, A549 cells, Oxidative stress, Apoptosis, Cyclins.


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Editor: Dr.Arvind Chavhan

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Cite this article as:

Kiran Kumar KM, Naveen Kumar M, Rashmi Nagesh, Patil Rajeshwari H, Babu RL, Sharma S Chidanandaand Manjunath K (2017) Arsenic induces oxidative stress and apoptosis in human lung cancer cells, International J. of Life Sciences, 5 (4): 551-560.

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Conflicts of interest: The authors stated that no conflicts of interest.

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Copyright: © 2017 | Author(s), This is an open access article under the terms of the Creative Commons Attribution-Non-Commercial - No Derivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Ahlemeyer B, Bauerbach E, Plath M, et al. (2001) Retinoic acid reduces apoptosis and oxidative stress by preservation of SOD protein level. Free Radic Biol Med, 30(10):1067-77


Babu RL, Naveen Kumar M, Patil RH, Devaraju KS, Ramesh GT, Sharma SC (2013) Effect of estrogen and tamoxifen on the expression pattern of AP-1 factors in MCF-7 cells: role of c-Jun, c-Fos, and Fra-1 in cell cycle regulation. Mol Cell Biochem, 380(1-2):143-51 doi:10.1007/ s11010-013-1667-x


Bertin G, Averbeck D (2006) Cadmium: cellular effects, modifications of biomolecules, modulation of DNA repair and genotoxic consequences (a review). Biochimie, 88(11):1549-59 doi:10.1016/j.biochi.-2006.10.001


Bouaziz H, Sefi M, de Lapuente J, Borras M, Zeghal N (2015) In vitro Cytotoxic and Genotoxic Effects of Arsenic Trioxide on Human Keratinocytes. World Academy of Science, Engineering and Technology, International Journal of Medical, Health, Biomedical, Bioengineering and Pharmaceutical Engineering, 9(3):231-234


Chakraborti D, Rahman MM, Paul K, et al. (2002) Arsenic calamity in the Indian subcontinent - What lessons have been learned? Talanta, 58(1):3-22 doi:Pii S0039-9140(02)00270-9


Chen CJ, Chen CW, Wu MM, Kuo TL (1992) Cancer potential in liver, lung, bladder and kidney due to ingested inorganic arsenic in drinking water. Br J Cancer, 66(5):888-92


Chou WC, Jie C, Kenedy AA, Jones RJ, Trush MA, Dang CV (2004) Role of NADPH oxidase in arsenic-induced reactive oxygen species formation and cytotoxicity in myeloid leukemia cells. Proc Natl Acad Sci U S A, 101(13):4578-83 doi:10.1073/pnas.0306687101


Flora SJ, Bhadauria S, Kannan GM, Singh N (2007) Arsenic induced oxidative stress and the role of antioxidant supplementation during chelation: a review. J Environ Biol, 28(2 Suppl):333-47


Frumkin H, Thun MJ (2001) Arsenic. Ca-a Cancer Journal for Clinicians, 51(4):254-262


Hong YS, Song KH, Chung JY (2014) Health effects of chronic arsenic exposure. J Prev Med Public Health, 47(5):245-52 doi:10.3961/jpmph.14.035


Hughes MF (2002) Arsenic toxicity and potential mechanisms of action. Toxicol Lett, 133(1):1-16


Hughes MF, Beck BD, Chen Y, Lewis AS, Thomas DJ (2011) Arsenic exposure and toxicology: a historical perspective. Toxicol Sci, 123(2):305-32 doi:10.1093/toxsci/kfr184


Hughes MF, Del Razo LM, Kenyon EM (2000) Dose-dependent effects on tissue distribution and metabolism of dimethylarsinic acid in the mouse after intravenous administration. Toxicology, 143(2):155-166 doi:Doi 10.1016/S0300-483x(99)00169-9


IARC. ( 2004) Some drinking-water disinfectants and contaminants, including Arsenic Monographs on the evaluation of carcinogenic risks to humans., vol WHO 84, 223-6., Lyon,France, p p. 68-70


Jaishankar M, Tseten T, Anbalagan N, Mathew BB, Beeregowda KN (2014) Toxicity, mechanism and health effects of some heavy metals. Interdisciplinary Toxicology, 7(2):60-72 doi:10.2478/intox-2014-0009


Kankofer M (2002) Superoxide dismutase and glutathione peroxidase activities in bovine placenta : spectrophotometric and electrophoretic analysis. . Revue Méd Vét, 153(2):121-124


Kapaj S, Peterson H, Liber K, Bhattacharya P (2006) Human health effects from chronic arsenic poisoning--a review. J Environ Sci Health A Tox Hazard Subst Environ Eng, 41(10):2399-428 doi:10.1080/10934520600873571


Kim TS, Jeong DW, Yun BY, Kim IY (2002) Dysfunction of rat liver mitochondria by selenite: induction of mitochondrial permeability transition through thiol-oxidation. Biochem Biophys Res Commun, 294(5):1130-7 doi:10.1016/S0006-291X(02)00612-5


Kiran Kumar KM, Naveen Kumar M, Patil RH, et al. (2016) Cadmium induces oxidative stress and apoptosis in lung epithelial cells. Toxicol Mech Methods, 26(9):658-666 doi:10.1080/15376516.2016.1223240


Mayer JE, Goldman RH (2016) Arsenic and skin cancer in the USA: the current evidence regarding arsenic-contaminated drinking water. International Journal of Dermatology, 55(11):E585-E591 doi:10.1111/ijd.13318

Miller WH, Jr., Schipper HM, Lee JS, Singer J, Waxman S (2002) Mechanisms of action of arsenic trioxide. Cancer Res, 62(14):3893-903


Moon KA, Guallar E, Umans JG, et al. (2013) Association Between Exposure to Low to Moderate Arsenic Levels and Incident Cardiovascular Disease. Annals of Internal Medicine, 159(10):649-+ doi:10.7326/0003-4819-159-10-201311190-00719

Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods, 65(1-2):55-63


Nagesh R, Kiran Kumar KM, Naveen Kumar M, et al. (2017) Aqueous areca nut extract induces oxidative stress in human lung epithelial A549 cells: Probable role of p21 in inducing cell death. Gene Reports, 6(Supplement C):103-111 doi:https://doi.org/10.1016/j.genrep.2016.12.008


Neganova I, Lako M (2008) G1 to S phase cell cycle transition in somatic and embryonic stem cells. J Anat, 213(1):30-44 doi:10.1111/j.1469-7580.2008.00931.x


Oh SH, Lee BH, Lim SC (2004) Cadmium induces apoptotic cell death in WI 38 cells via caspase-dependent Bid cleavage and calpain-mediated mitochondrial Bax cleavage by Bcl-2-independent pathway. Biochem Pharmacol, 68(9):1845-55 doi:10.1016/j.bcp.2004.06.021


Periyakaruppan A, Kumar F, Sarkar S, Sharma CS, Ramesh GT (2007) Uranium induces oxidative stress in lung epithelial cells. Archives of Toxicology, 81(6):389-395 doi:10.1007/s00204-006-0167-0


Periyakaruppan A, Sarkar S, Ravichandran P, et al. (2009) Uranium induces apoptosis in lung epithelial cells. Arch Toxicol, 83(6):595-600 doi:10.1007/s00204-008-0396-5


Pettersson HM, Karlsson J, Pietras A, Ora I, Pahlman S (2007) Arsenic trioxide and neuroblastoma cytotoxicity. J Bioenerg Biomembr, 39(1):35-41 doi:10.1007/s10863-006-9058-6


Ramirez J, Periyakaruppan A, Sarkar S, Ramesh GT, Sharma SC (2014) Effect of Simulated Microgravity on the Activity of Regulatory Enzymes of Glycolysis and Gluconeogenesis in Mice Liver. Microgravity Science and Technology, 25(5):303-309 doi:10.1007/s12217-013-9356-7


Schmidt CW (2014) Low-dose arsenic: in search of a risk threshold. Environ Health Perspect, 122(5):A130-4 doi:10.1289/ehp.122-A130


Sharma SC, Richards JS (2000) Regulation of AP1 (Jun/Fos) factor expression and activation in ovarian granulosa cells. Relation of JunD and Fra2 to terminal differentiation. J Biol Chem, 275(43):33718-28 doi:10.1074/jbc.M003555200


Shen HM, Yang CF, Ding WX, Liu J, Ong CN (2001) Superoxide radical-initiated apoptotic signalling pathway in selenite-treated HepG(2) cells: mitochondria serve as the main target. Free Radic Biol Med, 30(1):9-21


Shen ZY, Shen J, Cai WJ, Hong CQ, Zheng MH (2000) The alteration of mitochondria is an early event of arsenic trioxide induced apoptosis in esophageal carcinoma cells. International Journal of Molecular Medicine, 5(2):155-158


Smedley PL, Kinniburgh DG (2002) A review of the source, behaviour and distribution of arsenic in natural waters. Applied Geochemistry, 17(5):517-568 doi:Pii S0883-2927(02)00018-5


Tchounwou PB, Yedjou CG, Patlolla AK, Sutton DJ (2012) Heavy Metals Toxicity and the Environment. EXS, 101:133-164 doi:10.1007/978-3-7643-8340-4_6


Thomas D (1988) Handbook of Methods for Oxygen Radical Research. Journal of Pediatric Gastroenterology and Nutrition, 7(2):314-316


Tseng CH (2005) Blackfoot disease and arsenic: a never-ending story. J Environ Sci Health C Environ Carcinog Ecotoxicol Rev, 23(1):55-74 doi:10.1081/GNC-200051860


Waalkes MP, Liu J, Ward JM, Diwan BA (2004) Mechanisms underlying arsenic carcinogenesis: hypersensitivity of mice exposed to inorganic arsenic during gestation. Toxicology, 198(1-3):31-8 doi:10.1016/j.tox.2004. 01.017


WHO (2005) A field guide for detection, management and surveillance of arsenicosis cases. In: Editor. CD (ed). No 30, WHO, New Delhi: SEARO, p p. 5-18.


Yamanaka K, Takabayashi F, Mizoi M, An Y, Hasegawa A, Okada S (2001) Oral exposure of dimethylarsinic acid, a main metabolite of inorganic arsenics, in mice leads to an increase in 8-oxo-2 '-deoxyguanosine level, specifically in the target organs for arsenic carcinogenesis. Biochemical and Biophysical Research Communications, 287(1):66-70 doi:DOI 10.1006/bbrc. 2001.5551


Yedjou CG, Tchounwou PB (2007) In-vitro cytotoxic and genotoxic effects of arsenic trioxide on human leukemia (HL-60) cells using the MTT and alkaline single cell gel electrophoresis (Comet) assays. Mol Cell Biochem, 301(1-2):123-30 doi:10.1007/s11010-006-9403-4

 

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