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ISSN: 2320-964X (Online) 

ISSN: 2320-7817  (Print)



Dr. Santosh Pawar 




Int. Journal of Life Sciences, 2017; 5(4): 551-560             |        Available online, December 05, 2017

Arsenic induces oxidative stress and apoptosis in human lung cancer cells



Kiran Kumar KM1*, Naveen Kumar M1, Rashmi Nagesh1, Rajeshwari H Patil1, Babu RL 2.,

S Chidananda Sharma1and Manjunath K1

1Department of Microbiology and Biotechnology, Bangalore University, Jnana Bharathi, Bengaluru- 560 056, Karnataka, India

2Department of Bioinformatics and Biotechnology, Akkamahadevi Women’s University, Jnana Shakthi Campus, Vijayapura, 586 108, Karnataka, India

*Corresponding author e-mail-


Received: 06.10.2017   |    Accepted: 20.11.2017   |  Published : 05.12.2017

Arsenic is a toxic heavy metal and a well-known environmental contaminant. Often water, food, soil are found to be contaminated with arsenic and occupational exposure leads to harmful health issues. Hence, in the present study human lung epithelial A549 cells were used as in vitro model system. Treatment of A549 cells with arsenic for 48 h showed reduced viability with increased leakage of lactate dehydrogenase with loss of membrane damage. Arsenic significantly induces reactive oxygen species with depletion of free radical neutralizing antioxidant glutathione peroxidase and catalase enzymes. IC50 value of 2 µM arsenic concentration was used for mRNA expression studies. Further, pro-apoptotic Bax gene was induced and anti-apoptotic Bcl-2 was decreased. Arsenic stimulates dysfunction of cell cycle progression by downregulation of Cyclin-E1and Cyclin-D1 mRNA expressions. Thus confirms the toxic nature of arsenic and clues to oxidative redox potential alteration with cell cycle inhibition and apoptosis in lung epithelial cells.


Key words: Arsenic, A549 cells, Oxidative stress, Apoptosis, Cyclins.


Editor: Dr.Arvind Chavhan


Cite this article as:

Kiran Kumar KM, Naveen Kumar M, Rashmi Nagesh, Patil Rajeshwari H, Babu RL, Sharma S Chidanandaand Manjunath K (2017) Arsenic induces oxidative stress and apoptosis in human lung cancer cells, International J. of Life Sciences, 5 (4): 551-560.


Conflicts of interest: The authors stated that no conflicts of interest.



Copyright: © 2017 | Author(s), This is an open access article under the terms of the Creative Commons Attribution-Non-Commercial - No Derivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.



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