Image description
Image description

Published yearly: 

4 Issues

______________________

ISSN: 2320-964X (Online) 

ISSN: 2320-7817  (Print)

______________________

Editor-in-Chief

Dr. Santosh Pawar 


INDEXED IN

ORIGINAL ARTICLE

___________________________________________________________________________________

Int. Journal of Life Sciences, 2017; 5(4): 570-576             |        Available online, December 05, 2017

In silico structural characterization of Monooxygenase and dioxygenase enzymes from Danio rerio  


Chittaranjan Baruah1,* and Papari Devi2

 

1Department of Zoology, Darrang College, Tezpur – 784001, Assam, India

2Department of Zoology, Kaliabor College, Kuwarital-782137, Assam, India

*Corresponding author: chittaranjan_21@yahoo.co.in


Received: 05.10.2017   |   Accepted: 01.12.2017    |    Published : 05.12.2017

Freshwater fish having both retind and dehydretind showed the capabilities of clearing the provitamin A-Status carotenoids into both forms of Vitamin A, either through central or terminal cleavage. The involvement of monooxygenase and dioxygenase enzymes system has been identified. The present manuscript is presenting an in silico analysis that has been performed for molecular modeling and functional annotation of Monoxygenase and Dioxygenase enzymes from Danio rerio using sequence data extracted from publicly available database. The functional annotations have been performed using several publicly available Bioinformatics tools and databases. The study represents the application of comparative modeling method for 3D structure prediction. The functional annotations have been performed using several publicly available Bioinformatics tools and databases.

 

Keywords: Beta-carotene, Vitamin A, Homology modelling, phylogeny, ortholog, paralog

_________________________________________________________________________

Editor: Dr.Arvind Chavhan

___________________________________________________________________________________ 

Cite this article as:

Chittaranjan Baruahand Papari Devi (2017) In silico structural characterization of Monooxygenase and Dioxygenase enzymes from Danio rerio; International J. of Life Sciences, 5 (4): 570-576.

 

____________________________________________________________________________

Acknowledgement

The paper is dedicated with deep reverence to Late Professor UC Goswami, Former Professor and Head of Department of Zoology, Gauhati University, Assam, India who inspired the authors for molecular and in-silico analysis of Retinoids and Carotinoids.

____________________________________________________________________________

Conflicts of interest: The authors stated that no conflicts of interest.

____________________________________________________________________________

 

Copyright: © 2017 | Author(s), This is an open access article under the terms of the Creative Commons Attribution-Non-Commercial - No Derivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

_________________________________________________________________________

 

Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W and Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25(17):3389-402.


Altschul, Stephen F (1991) Amino acid substitution matrices from an information theoretic perspective .J. Mol. Biol.  219: 555-565.


Barua AB and Goswami UC (1977) Formation of vitamin A in a freshwater fish. Biochem. J. 164:133-136.

Barua AB, Singh HT, Das RC (1973) Conservation of lutein into dehydroretinol by the freshwater fish, Saccobranchus fossilis. Br. J. Nutr. 30: 1-12.


Eswar N, Eramian D, Webb B, Shen M, Sali A (2006) Protein Structure Modeling With MODELLER. in press

Fiser A, Do RK and Sali A (2000) Modeling of loops in protein structures, Protein Science 9: 1753-1773.


Giorgetti A, Raimondo D, Miele AE, Tramontano A (2005) Evaluating the usefulness of protein structure models for molecular replacement Bioinformatics, 21(2): 72–76.


Goswami UC and Barua AB (1981) Distribution of retinol and dehydroretinol in freshwater fish. Indian J. Biochem. Biophys. 18:383-385.


Higgins D, Thompson J, Gibson T, Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressivemultiple sequence alignment through sequence weighting,position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:4673-4680.


Laskowski RA, Watson, J.D., Thornton, J.M. (2003) From protein structure to biochemical function? J. Struct. Funct. Genomics, 4, 167–177


Marti-Renom MA, Stuart A, Fiser A, Sánchez R, Melo F and Sali A (2000) Comparative protein structure modeling of genes and genomes. Annu. Rev. Biophys. Biomol. Struct. 29: 291-325


Pittersen EF, Goddard TD, Huang CC, Couch GS, Greenblatt DM, Merg EC and Ferrin TE (2004) UCSF Chimera-a visualization system for exploratory research and analysis. J. Comput. chem.., 25:1605-1612.


Ramachandran & Sasisekharan (1968) The Ramachandran plot (Ramachandran & Sasisekharan) Adv. Protein Chem. 23: 283-437.
Redmond TM, Gentleman S, Duncan T, Yu S, Wiggert B, Gantt E, Cunningham FX Jr. (2001) Identification, expression, and substrate specificity of a mammalian beta-carotene 15,15'-dioxygenase. J Biol Chem 2; 276(9):6560-5. 

Saitou N and Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Evol. Biol 4(4):406-425.


Tiziana Castrignanò, Paolo D'Onorio De Meo, Domenico Cozzetto1, Ivano Giuseppe Talamo1 and Anna Tramontano, (2006) The PMDB Protein Model Database CASPUR, Nucleic Acids Research 2006 34(Database Issue): D306-D309; doi: 10.1093/nar/gkj105 Nucleic Acids Research 34, Database issue D306-D309.


Zemla A, Venclovas C, Moult J, Fidelis K (1999) Processing, and analysis of CASP3 protein structure predictions Proteins, Suppl. 3: 22–29.


Tamura K, Dudley J, Nei M & Kumar S (2007) MEGA4: Molecular Evolutionary (Multiple Sequence Alignment file has been given in the supplementary).

 

IJLSCI EVENTS

ADVERTISEMENT

 
















    INTERNATIONAL JOURNAL OF

    LIFE SCIENCES

    Print ISSN : 2320-7817 

    Online ISSN:2320-964X

    UGC Approved Journal No. 48951


    EDITORIAL OFFICE

    46, Guruwandan, Jawahar Nagar, 

    VMV Road, Amravati- 444604

    Maharashtra, India.

    Tel  + 91- 9970559438

    Email: editor@ijlsci.in | editorijlsci@gmail.com