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ISSN: 2320-964X (Online) 

ISSN: 2320-7817  (Print)

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RESEARCH ARTICLE

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Int. Journal of Life Sciences, 2018; 6(1): 111-116       |                 Available online, February 25, 2018

Effectiveness and efficiency of mutagen on leaf chlorophyll in Hyacinth bean Lablab purpureus

(L.) Sweet

 

Undirwade GP and Kulkarni GB

 

1Research Fellow; 2Assistant professor in Botany, J.E.S. R.G. Bagdia Arts, S.B. Lakhotia Commerce and R. Bezonji Science College, Jalna- 431203 (M.S.), India.

*Correspondence author:dr.kgb98@gmail.com

Received : 28.11.2017    |    Accepted : 12.02.2018        |        Published : 25.02.2018

The seeds of Hyacinth bean (Lablab purpureus L.) Sweet varieties Konkan bhushan and Konkan Wal-2 were treated with various doses of chemical (EMS- 0.05%, 0.10%, 0.15% and SA- 0.010%, 0.015%, 0.020%) and physical (Gamma rays- 5KR, 10KR, 15KR) mutagen and were sown in the field with control and harvested in bulk to raise M1 generation to observe the characters and number of mutants in each population. to observe the chlorophyll mutation and leaf morphology. Chlorophyll mutation frequency was calculated on the basis of plant population. A distinct range of chlorophyll and morphological mutants were observed in the M1 generations. Four different types of spectrum of chlorophyll mutant’s viz. albina, xantha, viridis and chlorina were identified in the treated population. Various morphological aberrations had been observe viz. Bilobed leaf, reduction in size of leaves, cotton shape leaf, obtuse and retuse apex, notch at margin, sinuate leaf margin, lanceolate leaf, wrinkled leaflet and triforked leaf. The frequency of leaf morphological changes noticed more in SA (0.020%) in Konkan bhushan and EMS (0.15%) in Konkan Wal-2 of Hyacinth bean variety.

 

Keywords: Hyacinth bean (Lablab purpureus L.) Sweet, EMS, SA, Gamma rays, chlorophyll mutation, leaf abnormalities


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Editor: Dr.Arvind Chavhan

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Cite this article as:

Undirwade GP and Kulkarni GB(2018) Effectiveness and efficiency of mutagen on leaf chlorophyll in Hyacinth bean Lablab purpureus (L.) Sweet, Int. J. of. Life Sciences, Volume 6(1): 111-116. 

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Conflicts of interest: The authors stated that no conflicts of interest.

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Copyright: © 2018 | Author(s), This is an open access article under the terms of the Creative Commons Attribution-Non-Commercial - No Derives License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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