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Published yearly: 

4 Issues

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ISSN: 2320-964X (Online) 

ISSN: 2320-7817  (Print)

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Editor-in-Chief

Dr. Santosh Pawar 


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RESEARCH ARTICLE

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Int. Journal of Life Sciences, 2018; 6(1): 143-149      |                 Available online, March 06, 2018

THP1 cell proteome library generation using SWATH analysis.


Hamza Baseerat1, 5*, Kumar Ajay1, 2, Midha Mukul1, 3, Sharma Anil1,4,

Sharma Bupender5 and Rao Kanury1, 2.

 

 

1International Center for Genetic Engineering and Biotechnology, New Delhi 110067

2Translational Health Science and Technology Institute, Faridabad, Haryana 121001.

3Institute for Systems Biology, 401 Terry Avenue North, Seattle, WA.

4Biological Innovation Research &Development Society. Janak Puri, New Delhi 110058.

5Mewar University, Chittorgarh, Rajasthan, 312901.

*Corresponding author shawlbaseerat@gmail.com

  

Received : 30.11.2017    |   Accepted : 03.02.2018       |      Published : 06.03.2018

THP-1 cell line is considered as an important model cell line to study the biology of the monocytes/ macrophages. The cell line is used to study the progression of various diseases such as, diabetes, tuberculosis and cancer. Advent of proteomics has revolutionized the discovery of new proteins by data independent acquisition (DIA) approach as well as the diagnosing the role of the novel or already known proteins in the cell biology by data dependent acquisition (DDA) approach. Recently, Sequential window acquisition of all theoretical mass spectra (SWATH) is introduced as a new Proteomics approach that carries the advantages of both the DIA as well as the DDA.Therefore a reference library has been  generated  for SWATH analysis and presented in the manuscript.

 

Key words: Macrophages, Mass Spectrometry, Proteins, Reference Library, SWATH analysis, Thp1 cell line.

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Editor: Dr.Arvind Chavhan

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Cite this article as:

Hamza Baseerat, Kumar Ajay, Midha Mukul, Sharma Anil, Sharma Bupender and Rao Kanury(2018) THP1 cell proteome library generation using SWATH analysis, Int. J. of. Life Sciences, Volume 6(1): 143-149 

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Conflicts of interest: The authors stated that no conflicts of interest.

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Copyright: © 2018 | Author(s), This is an open access article under the terms of the Creative Commons Attribution-Non-Commercial - No Derives License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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