Image description

Published yearly: 

4 Issues

______________________

ISSN: 2320-964X (Online) 

ISSN: 2320-7817  (Print)

______________________

Editor-in-Chief

Dr. Santosh Pawar 


INDEXED IN

RESEARCH ARTICLE

_______________________________________________________________________________________

Int. Journal of Life Sciences, 2018; 6(1): 6-12       |                 Available online, January 07, 2018

Study of Anti-psoriatic activity and Evaluation of E2A Gene Expression for Psoriasis Levels by RT-PCR of Thespesia populnea L. Methanolic Leaf Extract

Narendar Vankudothu1*, Chandrashekhar R2, Sudhakar Chekuri1, Lakshmi Bhavani N2 and Anwar SY 1

 

1Department of Genetics, University College of Science, Osmania University, Hyderabad, Telangana-500007, India.

2Pharmacognosy and Plant Molecular genetics Lab, Department of Botany, University College of Science, Saifabad, Osmania University, Hyderabad, Telangana-500004, India.

*Corresponding Author Email:  naren.genes@gmail.com, rupavathchandrashekhar@gmail.com

Received: 05.09.2017   |   Accepted: 29.12.2017    |    Published : 07.01.2018

Thespesia populnea L. (Malvaceae) traditionally claimed to be constructive in the cure of cutaneous infections such as scabies, psoriasis, ringworm, guinea worm, eczema and herpetic diseases. Oil prepared by boiling the ground bark and leaves in coconut oil is applied externally in psoriasis and scabies. Conversely there are no customary scientific reports for its anti-psoriatic activity. Hence the plant Thespesia populnea has been chosen to establish scientific data for its traditional claim as anti-psoriatic. In the present study a chemical test performed against all the phytochemical constituents for their preliminary screening of each solvent extract of leaf like water, methanol, chloroform, ethyl acetate, pet ether and acetone. From the phytochemical analysis of the leaf extracts; the result infers the presence of glycosides, tannins, flavonoids, triterpenoids and phytosterols in all the solvent extracts. But phenols are more retained in methanolic leaf extract compared to all the phytochemical constituents. Screening for anti-psoriatic activity was carried out by topical application of methanolic leaf extract of Thespesia populnea in the form of a cream using the Perry’s scientific mouse tail model. Successive methanolic leaf extract showed paramount anti-psoriatic activity than the standard drug Retion-A (0.05%). Potential function of E2A as a biomarker in psoriasis, is applied in the current investigation to study whether the methanolic leaf extracts of Thespesia populnea L. showed antipsoriatic effect targeting E2A gene expression for the psoriasis levels.   Results indicated that methanolic leaf extract of Thespesia populnea can preferably be used as anti-psoriatic drug and the plant is promising for further investigations to prove its anti-psoriatic activity with no side effects.

 

Keywords: Thespesia populnea L. Anti-psoriatic activity, Phytoconsituents, Retion-A (0.05%)

 

_________________________________________________________________________

Editor: Dr.Arvind Chavhan

___________________________________________________________________________________ 

Cite this article as:

Narendar Vankudothu, Chandrashek-har R, Sudhakar Chekuri, Lakshmi Bhavani N and Anwar SY (2018) Study of Antipsoriatic activity and Evaluation of E2A Gene Expression for Psoriasis Levels by RT-PCR of Thespesia Populnea L. Methanolic Leaf Extract; International J. of Life Sciences, 6 (1): 6-12. 

____________________________________________________________________________

Acknowledgements

Authors are highly acknowledged to University Grants Commission (UGC) Government of India for funding this project in the form of Research Fellowship for Meritorious Students (RFSMS-FELLOWSHIP).Authors acknowledged to Head, Department of Genetics, University College of Science, Osmania University, Hyderabad, Telangana, 500007 for providing lab facility to complete this work.Authors express their sincere thanks to all the fellows who helping in the procurement of plant material    from source arrears in the present work. Authors are highly acknowledged to Central Facilities for Research and Development (CFRD) Osmania University, Hyderabad, Cinteny Lab and Jeeva life sciences Bio Labs for support of instrumentation to complete this work.

_________________________________________________________________________________________ 

Conflict of Interest/ Author Contributions

Prof.S.Y.Anwar designed the work, Narendar.V performed studies, Sudhakar Chekuri and Dr.R. Chandrashekhar helped the data interpretations. All the authors reviewed the manuscript.

____________________________________________________________________________

 

Copyright: © 2018 | Author(s), This is an open access article under the terms of the Creative Commons Attribution-Non-Commercial - No Derives License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

_________________________________________________________________________

 

Akindele AJ, Adeyemi OO (2007) Anti inflammatory activity of the aqueous leaf extract of Byrsocarpuscoccineus. Fitoterapia 2007, 78(1):25-8.


Bjorntorp  E,  Parsa  R,  Thornemo  M,  Wennberg  AM  et  al.   (2003) The helix-loop-helix transcription factor Id1 is highly expressed in psoriatic involved skin. Acta Derm. Venereol., 83: 403-409.


Chandran RP, Manju S, Shaji PK, Nair GA and Sukumar B (2016) In Vitro Cytotoxic Activities of Leaf Extracts of Thespesia populnea and HygrophillaSchulli Against Dalton’s Lymphoma Ascites and Ehrlich Ascites Carcino-ma Cell Lines. Austin J.Lung Cancer Res, 1(2): 1007.


Chikhali Umakant, Shankar Shinde, Amar Dinkar, Patil Sandip, Balvant Naikwade (2011) Evaluation of antitumor, antioxidant and cytotoxic activity of Thespesia populnea bark extract. Journal of Pharmacy Research, 4(10):3641


Engel I and Murre C (2001) The function of E- and Id proteins in lymphocyte development. Nat. Rev. Immunol. 1: 193- 199.


Karna P, Chagani S, Gundala SR, Rida PCG, Asif G, Sharma V, Gupta MV, Aneja R (2012) Benefits of whole ginger extract in prostate cancer. British Journal of Nutrition, 107(4):473–484.


Kee BL (2009) E and ID proteins branch out. Nat. Rev. Immunol., 9: 175-184.


Lindamulage IK, Soysa P (2016) Evaluation of anticancer properties of a decoction containing Adenanthera-pavonina L. and Thespesiapopulnea. BMC Complement Altern Med., 16:70


Liu RH (2003) Health benefits of fruit and vegetables are  from additive and synergistic combinations    of    phytochemicals.    American    Journal    of    Clinical    Nutrition.78(3):517S–520S.


Mark EB, Jonsson M, Asp J, Wennberg AM et al. (2006) Expression of genes involved in the regulation of p16 in psoriatic involved skin. Arch.Dermatol.Res.,297:459-467.


Patwardhan B, Vaidhya ADB and Chorghade M (2004) Ayurveda and Naturalproducts drug discovery. Curr Sci., 86: 789-799.


Perk J, Iavarone A and Benezra R (2005) Id family of helix-loop-helix proteins in cancer. Nat.Rev.Cancer.5:603-614.


Pritam Kumar Panda, Danish Ibrahim, Pramod Kumar P. Gupta (2014) Computational modeling and analysis of theoretical structure of corneodesmosin receptor protein with existing phytochemicals in psoriasis, Indian J. of Fund. and Applied Life Sciences.2014, 4 (4), 346-355.


Parthasarathy R, Ilavarasan R, Karrunakaran CM (2009) Antidiabetic  activity  of ThespesiaPopulnea bark and leaf extract against streptozotocin induced diabetic rats. International J. of Pharm.Tech Research. 2009.


Samuel ML, Donald PM, Hurley JH Jr (1986) Dermatology Vol-I. W. B. Philadelphia:SaundersCompany; p.204.


Sandhya T, Lathika KM, Pandey BN, Mishra KP (2006) Potential of ayurvedic formulation, Triphala, as novel anticancer drug. Cancer letters, 231(2): 206-214.


Satyavati GV, Gupta AK, Tandon N (1987) Medicinal Plants  of  India. ICMR, New Delhi, India.


Siddharth Shrivastav, Rakesh K. Sindhu, Sanjeev Kumar, Pradeep Kumar (2009) Anti-Psoriatic And Phytoche-mical Evaluation Of Thespesia Populnea Bark Extracts. Int J Pharm Pharm Sci


Si-Yuan Pan, Gerhard Litscher, Si-HuaGao, Shu-Feng Zhou, Zhi-Ling Yu, Hou-Qi Chen, Shuo-Feng Zhang, Min-Ke Tang, Jian-Ning Sun, and Kam-Ming Ko. Historical Perspective of Traditional Indigenous Medical Practices: The Current Renaissance and Conservation of Herbal  Resources. Evidence-Based Complementary and Alternative   Medicine. 2014.


Walter LF, Gundula S (1981) Histopathology of the skin. 3rd Edn., Boston, Massachusetts: Lippincott;p.156-64.


Yogayata S Pathare, Vijay D Wagh(2012) Herbal Medicines and Nutritional supplements used in the treatment of Glaucoma: A Review. RJPBCS,  Volume 3 Issue 1.

 

IJLSCI EVENTS

ADVERTISEMENT

 
















    INTERNATIONAL JOURNAL OF

    LIFE SCIENCES

    Origin & Evolution

    Print ISSN : 2320-7817 

    Online ISSN:2320-964X

    UGC Approved Journal No. 48951


    EDITORIAL OFFICE

    46, Guruwandan, Jawahar Nagar, 

    VMV Road, Amravati- 444604

    Maharashtra, India.

    Tel  + 91- 9970559438  |   9420775527  

    Email: editor@ijlsci.in | editorijlsci@gmail.com