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Published yearly: 

4 Issues

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ISSN: 2320-964X (Online) 

ISSN: 2320-7817  (Print)

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Editor-in-Chief

Dr. Santosh Pawar 


INDEXED IN

RESEARCH ARTICLE

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Int. Journal of Life Sciences, 2018; 6(1): 166-172       |                 Available online, March 09, 2018

Acute toxicity of triclosan on the native freshwater fish, Anabas testudineus (Bloch,1792): behavioral alterations and histopathological lesions


Chokki Veettil Priyatha and Kumari Chidambaran Chitra*


 

Endocrinology and Toxicology Laboratory, Department of Zoology, University of Calicut, Malappuram District, Kerala, India-673635

*Corresponding author Email: kcchitra@yahoo.com


Received : 20.10.2017     |      Accepted : 01.02.2018        |      Published : 09.03.2018

Triclosan is an effective antimicrobial agent commonly used in pharmaceuticals and personal care products. In this study, median lethal concentration i.e., 96h-LC50 value, along with behavioural and histopathological modifications of triclosan were investigated in the native fish, Anabas testudineus. For determining LC50-96h of triclosan, seven different concentrations of the test chemical at a range from 1 to 2.2 mg/L in a geometric series were selected after a range-finding test. Control group without the test solution and the positive control containing the solubilising agent (DMSO) as a vehicle was also maintained, comprising ten animals per group. The median lethal concentration of triclosan in Anabas testudineus estimated by using Probit analysis was 1.767 mg/L. Behavioural modifications noted during the study includes air gulping, surfacing, reduced opercular movement, mucous deposition, bulged and hemorrhagic eyes. Histopathological alterations observed in gill consist of uplifting and hyperplasia of epithelium, aneurysm, lamellar disorganization as shortened primary lamella, fusion or loss of secondary lamella and hyperplasia of gill arches. Hepatocytes showed histological alterations such as irregular or anucleated hepatocytes, cytoplasmic vacuolization, degenerated cytoplasm and aggregation of melanomacrophages. The intensity of morphological lesions increases when the concentration is increased and also substantiates the behavioural alterations exhibited by the fish during triclosan exposure. From the result of probit statistical method, it is evident that the acute toxic effect of triclosan is concentration-dependent, and therefore, triclosan contamination is a threat to aquatic organisms.

 

Keywords: Triclosan, Anabas testudineus, 96h-LC50, Behaviour, Histopathology

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Editor: Dr.Arvind Chavhan

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Cite this article as:

Chokki Veettil Priyatha and Kumari Chidambaran Chitra (2018) Acute toxicity of triclosan on the native freshwater fish, Anabas testudineus (Bloch, 1792): behavioral alterations and histopathological lesions, Int. J. of. Life Sciences, Volume 6(1): 166-172. 

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Acknowledgement

The authors are grateful to the financial support from KSCSTE, Thiruvananthapuram, Kerala, India for the present study.

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Conflicts of interest: The authors stated that no conflicts of interest.

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Copyright: © 2018 | Author(s), This is an open access article under the terms of the Creative Commons Attribution-Non-Commercial - No Derives License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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