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Published yearly: 

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ISSN: 2320-964X (Online) 

ISSN: 2320-7817  (Print)

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RESEARCH ARTICLE

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Int. Journal of Life Sciences, 2018; 6(1): 181-186       |                 Available online, March 13, 2018

Micropropagation of the Monopodial Orchid,   Rhynchostylis retusa (L.)


Sunitibala Devi Y and Neelashree  N

 

Advanced Level Institutional Biotech-Hub, Department of Botany, G.P. Women’s College, Imphal, Manipur, India.

 

Received : 23.11.2017    |    Accepted : 07.03.2018      |     Published : 13.03.2018

Rhynchostylis retusa, a monopodial orchid is important for both its medicinal and economic values. Different parts like shoot, leaf and root were used as explants for micropropagation of Rhynchostylis retusa. The explants were cultured in Vacin and Went (VW), Knudson C and Nitsch media supplemented with sucrose (20 g L-1) and growth regulators, viz., NAA (0.1 mgL-1), BAP (1.0 mgL-1) and Kinetin, Kn (0.4-1.0 mgL-1) in different concentrations and combinations. Green protocorm-like bodies (PLBs) and calli which later differentiated into plantlets were induced from shoot-tip, leaf and root-tip explants using VW medium supplemented with NAA (0.1 mgL-1) along with either Kinetin (1.0 mgL-1) or BAP (1.0 mgL-1). Maximum survival as well as differentiation was obtained from shoot-tip explants. Further growth and development of PLBs into plantlets were achieved when cultured in VW medium with gradual removal of NAA and reduction of Kinetin and BAP concentrations. Transplantation in community pots could be done when the plantlets were 3.0-3.5 cm. These results suggest a methodology for in vitro mass propagation of Rhynchostylis retusa.

 

Keywords: Micropropagation, Rhynchostylis retusa, PLBs (Protocorm-like bodies), in vitro, plantlets

 

 

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Editor: Dr.Arvind Chavhan

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Cite this article as:

Sunitibala Devi Y and Neelashree  N (2018) Micropropagation of the Monopodial Orchid, Rhynchostylis retusa (L.), Int. J. of. Life Sciences, Volume 6(1): 181-186. 

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Acknowledgement

The authors are thankful to the Department of Biotechnology, Ministry of Science and Technology, Government of India for providing working facility by giving financial assistance for the establishment of Institutional Level Biotech Hub.

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Conflicts of interest: The authors stated that no conflicts of interest.

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Copyright: © 2018 | Author(s), This is an open access article under the terms of the Creative Commons Attribution-Non-Commercial - No Derives License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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