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Published yearly: 

4 Issues

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ISSN: 2320-964X (Online) 

ISSN: 2320-7817  (Print)

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Editor-in-Chief

Dr. Santosh Pawar 


INDEXED IN

RESEARCH ARTICLE

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Int. Journal of Life Sciences, 2018; 6(1): 49-59       |                         Available online, February 24, 2018

Species relationships among wild and cultivated   Abelmoschus Medik., (Malvaceae) species as 

reveled by molecular markers


Patil Pravin1*, Sutar Shrikant2  and Bhat Kangila Venkataraman3

 

1Shankrlal Agrawal Science College, Salekasa, Dist. Gondia, India |  

2NowrosjeeWadia College, Pune, India

3National Bureau of Plant Genetic Resources, New Delhi, India

*Corresponding Author Email: pravin.patil99@gmail.com

Received : 23.09.2017    |     Accepted : 19.01.2018       |       Published : 24.02.2018

The genus Abelmoschus is represented by more than 10 species which shows wide distribution over the diverse agro−climatic conditions of Indian subcontinent. In the present study, total 60 accessions of eleven Abelmoschus species were subjected to estimate genetic diversity and species relationships. Out of 200 primers 21 primers was found to be considerable for cluster analysis. All 21 primers produced total 321 amplicons with ranges from nine (OPA-5) to nineteen (105, 966) and average number of amplicons detected were 15.3 per primer. Out of the 321 amplicons amplified, 301 were polymorphic showing 93.77% polymorphism with average of 14.3 per primer. Among the studied species, high genetic diversity was observed in A. manihot subsp. tetraphyllus var. tetraphyllus (0.1711), whereas lowest genetic diversity was observed in A. manihot subsp. tetraphyllus var. pungens. Among the studied species highest (0.9095) similarity index was observed between A. esculentus and A. caillei whereas A. palianus and A. crinitus exhibited lowest (0.5149) similarity index. The UPGMA based cluster analysis clearly distinguishes the Abelmoschus species into three major clusters – I, II and III.  This study validate the utility of RAPD markers as a reliable tool for phylogenetic relationships as well as effective characterization of Abelmoschus genetic resources.

 

Keywords: Abelmoschus, genetic diversity, okra, RAPD, species relationships

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Editor: Dr.Arvind Chavhan

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Cite this article as:

Patil Pravin, Sutar Shrikant and Bhat Kangila Venkataraman (2018) Species relationships among wild and cultivated Abelmoschus Medik., (Malvaceae) species as reveled by molecular markers, Int. J. of. Life Sciences, Volume 6(1): 49-59. 

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Conflicts of interest: The authors stated that no conflicts of interest.

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Copyright: © 2018 | Author(s), This is an open access article under the terms of the Creative Commons Attribution-Non-Commercial - No Derives License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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