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Published yearly: 

4 Issues

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ISSN: 2320-964X (Online) 

ISSN: 2320-7817  (Print)

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Editor-in-Chief

Dr. Santosh Pawar 


INDEXED IN

RESEARCH ARTICLE

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Int. Journal of Life Sciences, 2018; 6(2): 394-398       |                 Available online, April 25, 2018

Purification and Characterization of A Novel CGTase from Alkaliphilic  Bacillus species isolated from Lonar lake , India


Shinde VA1, Patil RB2 and Pawar PV3

 

1Asst. Professor, Department of Microbiology, 2Head, Department of Botany, Shankarrao Chavan Mahavidyalaya, Ardhapur, Nanded, India.

3Asst. Professor, Department of Botany, Madhavrao Patil College, Palam, Hingoli, India.

*Address for Correspondence: Dr. Shinde V. A., Asst. Professor, Department of Microbiology, Shankarrao Chavan Mahavidyalaya, Ardhapur, Nanded, India.  Phone No. : +91-9921214222, email: shinde_vinod21@yahoo.com

 

Received : 02.11.2017     |     Accepted : 18.03.2018        |         Published : 25.04.2018

Production and purification of Cyclodextrin glycosyltransferase (CGTase) from Alkaliphilic Bacillus species isolated from Lonar lake , India was investigated in present study. Production was carried out using medium containing starch, yeast extract, peptone MgSO4.7H2O. the crude enzyme was collected by centrifugation and partially purified using ammonium sulphate precipitation method. This partially purified enzyme was further purified using phenyl sepharose column chromatography. The enzyme obtained had molecular weight of 79.20 kDa which is confirmed by SDS PAGE and Mass spectroscopy.


Keywords: Cyclodextrin glycosyltransferase (CGTase); purification; Phenyl sepharose; column chromatography.

 

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Editor: Dr.Arvind Chavhan

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Cite this article as:

Shinde VA, Patil RB and Pawar PV (2018) Purification and Characterization of A Novel CGTase from Alkaliphilic  Bacillus species isolated from Lonar lake , India, Int. J. of. Life Sciences, Volume 6(1): 394-398.

 

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Conflicts of interest: The authors stated that no conflicts of interest.

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Copyright: © 2018 | Author(s), This is an open access article under the terms of the Creative Commons Attribution-Non-Commercial - No Derives License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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