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ISSN: 2320-964X (Online) 

ISSN: 2320-7817  (Print)

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RESEARCH ARTICLE

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Int. Journal of Life Sciences, 2018; 6(2): 420-426       |                 Available online, April 25, 2018

Isolation and Purification of antibacterial proteins from hemolymph of Marine gastropods dwelling at rocky shores of Kanyakumari, Southeast coast of India


Sharmin Vini S, Viju N, Ezhil Raj N and Mary Josephine Punitha S٭

 

Centre for Marine Science and Technology, Manonmaniam Sundaranar University, Marina Campus, Rajakkamangalam- 629502, Tamilnadu, India.

٭Corresponding author: S. Mary Josephine Punitha, e-mail: punithapeter10@gmail.com

 

Received : 14.11.2017   |    Accepted : 29.03.2018    |     Published : 25.04.2018

Proteins from body fluid (hemolymph) of two marine gastropods Nerita albicilla and Purpura bufo collected from the coastal regions of Kanyakumari were precipitated with ammonium sulphate and purified using ion-exchange chromatography. The fractions obtained were tested for their antibacterial potential against human pathogens. Fractions F2 and F4 from N.albicilla showed higher inhibitory activity against Salmonella typhi, Streptococcus mutans and Staphylococcus aureus. The molecular weight of this antibacterial protein present in the fractions was determined with the help of SDS PAGE and it was found to be 30KDa. Further, characterization of this active protein paves the way for the development of new antibiotic against harmful human pathogens in future.

 

Keywords: Gastropods, hemolymph, peptides, pathogens, antibiotics

 

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Editor: Dr.Arvind Chavhan

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Cite this article as:

Sharmin Vini S, Viju N, Ezhil Raj N and Mary Josephine Punitha S (2018) Isolation and Purification of antibacterial proteins from hemolymph of Marine gastropods dwelling at rocky shores of Kanyakumari, Southeast coast of India., Int. J. of. Life Sciences, Volume 6(2): 420-426. 

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Acknowledgements

We authors are thankful to INSPIRE Fellowship program, (DST) Department of Science and Technology, New Delhi for finance assistance and also to CMST, UGC- SAP and DST- FIST sponsored research department for providing lab facilities to carry out the work.  

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Conflicts of interest: The authors stated that no conflicts of interest.

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Copyright: © 2018 | Author(s), This is an open access article under the terms of the Creative Commons Attribution-Non-Commercial - No Derives License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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