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ISSN: 2320-964X (Online) 

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RESEARCH ARTICLE

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Int. Journal of Life Sciences, 2018; 6(2): 434-440       |                 Available online, April 25, 2018

Exploring the industrially important extracellular enzymes from different nematode-trapping fungi

Kunjadia Anju1, Darji Nikita**2, Patel Babulal4 and Pandit Ramesh*2,3

  

1Centre for Interdisciplinary Studies in Science and Technology (CISST), Sardar Patel University, Vallabh Vidyanagar, Anand-388 121, Gujarat, India.

2Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied Sciences (ARIBAS), ADIT Campus, New V.V. Nagar 388 121, Gujarat, India.

3Department of Animal Biotechnology, College of Veterinary Science and A.H., Anand Agricultural University, Anand-388 001, Gujarat, India.

4V.P. & R.P.T.P. Science College, Sardar Patel University, Vallabh Vidyanagar, Anand-388 120, Gujarat. (INDIA).

*Correspondence details: panditrameshj@gmail.com

**This author have equal contribution for this research work.

 

Received : 02.02.2018    |     Accepted : 29.03.2018     |     Published : 25.04.2018

Nematophagous fungi are potential bio-control agent against the plant and animal parasitic nematodes. The extracellular hydrolytic enzymes are one of the important virulence factors for these fungi. In the present study, we investigated industrially important extracellular enzymes from two different genera of nematode-trapping fungi. Duddingtonia flagrans produced the highest amount of amylase (261 U/mL/min) and xylanase (76mU/mL/min) compared to Arthrobotrys conoides (186 and 39 U/mL/min) while it was reversed in case of cellulase, highest (214 U/mL/min) in A. conoides. Protease, chitinase and collagenase activity (236, 19.83 and 2.46 U/mL/min) was higher during induction in D. flagrans RPAN-10 as compared to A. conoides (118.6, 8.3 and 1.2 U/mL/min). D. flagrans RPAN-10 showed 3.14 fold and A. conoides 2.37 fold change in protease activity when induced with nematodes. However, there was no apparent difference in control and induced fungus for chitinase and collagenase. In summary, D. flagrans RPAN-10 was found to be a suitable candidate for further exploring the protease, amylase, chitinase and xylanase while A. conoides is suitable for cellulase production for further application. However, further research is required in order to optimization of growth conditions as well as suitable nutrients in order for large scale production for industrial applications.

 

Key words: Nematophagousfungi. Arthrobotrys conoides, A. conides, Duddingtonia flagrans and D. flagrans. Bio-control, , Extracellular enzymes.

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Editor: Dr.Arvind Chavhan

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Cite this article as:

Kunjadia Anju, Darji Nikita, Patel Babulal and Pandit Ramesh (2018) Exploring the industrially important extracellular enzymes from different nematode-trapping fungi, Int. J. of. Life Sciences, Volume 6(1): 434-440.

 

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Acknowledgement:

The authors would like to thank Charutar Vidya Mandal (CVM), Vallabh Vidyanagar for providing platform and necessary support to carry out this research. 

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Conflicts of interest: The authors stated that no conflicts of interest.

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Copyright: © 2018 | Author(s), This is an open access article under the terms of the Creative Commons Attribution-Non-Commercial - No Derives License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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