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ISSN: 2320-964X (Online) 

ISSN: 2320-7817  (Print)

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RESEARCH ARTICLE

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Int. Journal of Life Sciences, 2018; 6(2): 326-334       |                 Available online, April 25, 2018

Assessment of contamination levels and characterization of Aflatoxin B1 producing Aspergillus flavus strain from food and feed samples from local markets of south India

 

Manjunatha AS*, Manjunath K and Mohana DC

 

Environment Microbiology laboratory, Department of Microbiology, Bangalore University, Bangalore-560056.

*Corresponding author E-mail: manjunathronur@gmail.com 

 

Received : 07.02.2018     |     Accepted : 21.03.2018        |         Published : 25.04.2018

Aflatoxins are the toxic secondary metabolites produced by fungi Aspergillus flavus and A. parasiticus in many food and feed products including oil seeds when stored under ambient conditions. Aflatoxin B1 a prototype of the aflatoxin is widely recognized as group I human hepato-carcinogenic compound by IARC. Contamination of oil seeds with the Aspergillus Sps and aflatoxin possess serious threat to the food and feed industry. The present study on the incidence of seed borne infection and evaluation of aflatoxin B1 contamination levels in oil seeds by applying the principles of standard blotter method and chromatography methods respectively. The molecular characterization of toxigenic isolate No.39 by PCR method. The percentage incidence of seed borne infection was observed to be higher for the groundnut sample showing the mean percentage of infection to be about 48.57% than with all the seed samples. Among 55 isolates of Aspergillus flavus, 42 were found to be aflatoxigenic strains by TLC and amount of aflatoxin B1 in oil seeds was found to be in the range of 29-35µg/ml by HPLC. The molecular characterization of toxigenic isolate was found to be closely related to Aspergillus flavusstrain DAOM 225949 and the nucleotide sequence was provided with a GenBank accession number JN938987. This study concluding that the oil seeds were prone to fungal infection and contamination with aflatoxin B1 when stored under ambient conditions. So, proper storage, improvement of Good Agricultural Practices and regular inspection should be practiced ensuring that the safety of animals including human beings.

 

Key words:  Food; Oil seeds; Aspergillus flavus; aflatoxin; TLC; HPLC; PCR; Phylogenetic analysis.

 

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Editor: Dr.Arvind Chavhan

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Cite this article as:

Manjunatha AS, Mohana DC and Manjunath K (2018) Assessment of contamination levels and characterization of aflatoxin B1 producing Aspergillus flavus strain from food and feed samples from local markets of south India, (Malvaceae) species as reveled by molecular markers, Int. J. of. Life Sciences, Volume 6(1): 326-334. 

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Conflicts of interest: The authors stated that no conflicts of interest.

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Copyright: © 2018 | Author(s), This is an open access article under the terms of the Creative Commons Attribution-Non-Commercial - No Derives License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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