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Published yearly: 

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ISSN: 2320-964X (Online) 

ISSN: 2320-7817  (Print)

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Dr. Santosh Pawar 


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RESEARCH ARTICLE

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Int. Journal of Life Sciences, 2018; 6(2): 540-543      |                 Available online, April 26, 2018

A simple and reliable method for obtaining monoconidial culture and storage of Magnaporthe oryzae 

 

Jagadeesh D1, Prasanna Kumar MK2 and Devaki NS1

 

1 Department of Molecular Biology, Yuvaraja’s College, University of Mysore, Mysuru 570005

2 Department of Plant Pathology, GKVK, University of Agricultural Science, Bangalore 560065

 Email ID: devakineerkaje@gmail.com   |   +91- 9448933006

Corresponding author Name: Dr. N. S. DEVAKI, Associate Professor and course coordinator, Department of  Molecular Biology, Yuvaraja’s College, University of Mysore, Mysuru 570 005

 

Received : 06.03.2018  |  Revised : 15.04.2018 |   Accepted : 23.04.2018   |  Published : 26.04.2018

Monoconidial isolates of Magnaporthe oryzae were successfully cultured and maintained using a simple, reliable and inexpensive isolation procedure developed by us. It can also be performed with minimum equipment. This procedure will be helpful for researchers to save their time in standardizing the isolation and storage. We have isolated 72 M. oryzae monoconidial isolates from different agro climatic regions of Karnataka, India and pure cultures were stored in filter papers, in our investigation. The current procedure is useful in carrying out research investigation requiring genetically uniform cultures.

 

Keywords: Magnaporthe oryzae; Monoconidial isolate; Rice blast disease

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Editor: Dr.Arvind Chavhan

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Cite this article as:

Jagadeesh D, Prasanna Kumar MK and Devaki NS (2018) A simple and reliable method for obtaining monoconidial culture and storage of Magnaporthe oryzae, Int. J. of. Life Sciences, Volume 6(2): 540-543. 

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Acknowledgements 

We thank University Grant Commission, New Delhi for the financial support by sanctioning Major Research Project (F.No. 41-408/2012 (SR) dated July 2012) to carry out this Investigation. We are also thankful to Yuvaraja’s College, University of Mysore, Mysuru for providing facilities for carrying out this research work

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Conflicts of interest: The authors stated that no conflicts of interest.

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Copyright: © 2018 | Author(s), This is an open access article under the terms of the Creative Commons Attribution-Non-Commercial - No Derives License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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