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Published yearly: 

4 Issues

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ISSN: 2320-964X (Online) 

ISSN: 2320-7817  (Print)

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Editor-in-Chief

Dr. Santosh Pawar 


INDEXED IN

RESEARCH ARTICLE

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Int. Journal of Life Sciences, 2018; 6(2): 563-568      |                 Available online, April 26, 2018

Evaluation of Phytochemical compounds by TLC and FT-IR analysis of Cipadessa baccifera (Roth) Miq. and Elytraria acaulis (L.f) Lindau

 

Jeevitha DS, Manjunath Kiragandur, Manjunath AS

 

 

Department of Microbiology and Biotechnology, Jnanabharathi Campus, Bangalore University,

Bangalore-560 056

Email: jeevithawelcomes@gmail.com | 9663783499

Received : 29.01.2018   |    Revised : 06.03.2018    |    Accepted : 16.04.2018   |    Published : 26.04.2018

The present study was carried out to investigate the medicinal bioactive substances present in the petroleum ether, toluene and methanolic extracts Cipadessa baccifera and petroleum ether and methanolic leaf extracts of Elytraria acaulis. In the present investigation, chromatographic techniques such as Thin Layer Chromatography (TLC) analysis was used to separate the bioactive compound from the crude leaf extracts of Cipadessa baccifera and Elytraria acaulis.  The solvent system of TLC was Methanol: Chloroform (1:1, 6:4, 7:3 and 9:1) used and Rf value was recorded. TLC chromatogram was subjected to bioautography to check the antibacterial activity against Staphylococcus aureus. For FTIR analysis the leaf extract of Cipadessa baccifera and Elytraria acaulis was focused in the transmittance ranging from 400-4000cm-1 on a Perkin Elmer Spectrum and the characteristic peak values and their functional groups were detected.  From TLC analysis spots were identified with Rf values 0.76 and 0.83 was compared with literature data showed that the presence of flavonoid compound as quercetin.  Bioautography showed zone of clearance indicates the presence of active components inhibits the growth of Staphylococcus aureus when the chromatogram is sprayed with p–iodonitrotetrazolium violet (INT) spray.  

 

Key words: Thin Layer Chromatography, FT-IR, Cipadessa baccifera, Elytraria acaulis.

 

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Editor: Dr.Arvind Chavhan

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Cite this article as:

Jeevitha DS, Manjunath Kiragandur, Manjunath AS (2018) Evaluation of Phytochemical compounds by TLC and FT-IR analysis of Cipadessa baccifera (Roth) Miq. and Elytraria acaulis (L.f) Lindau, Int. J. of. Life Sciences, Volume 6(2): 563-568. 

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Conflicts of interest: The authors stated that no conflicts of interest.

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Copyright: © 2018 | Author(s), This is an open access article under the terms of the Creative Commons Attribution-Non-Commercial - No Derives License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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    INTERNATIONAL JOURNAL OF

    LIFE SCIENCES

    Origin & Evolution

    Print ISSN : 2320-7817 

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