Image description

Published yearly: 

4 Issues

______________________

ISSN: 2320-964X (Online) 

ISSN: 2320-7817  (Print)

______________________

Editor-in-Chief

Dr. Santosh Pawar 


INDEXED IN

RESEARCH ARTICLE

_______________________________________________________________________________________

Int. Journal of Life Sciences, 2018; 6(2): 688-699      |                 Available online, April 28, 2018

Estimation of reserpine from healthy and diseased roots of Rauwopfia serpentina (L.) Benth. Ex. Kurz (Sarpagantdha) from different isolates by HPTLC analytical method

 

Dudhbhate MM1  and  Kareppa BM2

 

1Dept of Botany, ACS College, Gangakhed, MS, India

2Dept. of Botany, DSM College, Parbhani-431401.

*Corresponding author email: mmdudhbhate@rediffmail.com  | kareppabm@gmail.com

   

Received : 16.01.2018     |    Revised : 31. 03.2018  |   Accepted : 23.04.2018   |   Published : 28.04.2018

Rauwolfia serpentina is an important medicinal herb used in Ayurveda and Alleopathy. Reserpine is an indole alkaloid present in Rauwolfia serpentina viz. reported to possess anti-hypertensive and tranquilizer property. Evaluation of herbal drug based on the amount of active constituent. Reserpine is present in all plant parts, but more in roots. Various factors are responsible for growth of plants and active constituent present in it. Roots are infected by fungi causing root rot disease that affect active constituent of root. Among the fungi, Macrophomina phaseolina causes severe root rot disease .In order to Changes in reserpine from healthy and infected roots of Rauwopfia serpentina, the healthy and infected roots of Sarpagandha was collected from four different places namely M. A., University, Parbhani designated HRS-1, IRS-1, Medicinal plant garden, M.P.K.V., Rahuri, designated HRS-2, IRS-2, Nagarjun medicinal plant garden P. D. K. V., Akola designated HRS-3, IRS-3 and S. G. M., Amravati University, Amravati designated HRS-4, IRS-4 during rainy season in the month of August 2009 was used for the analysis. In the present study, estimation of reserpine from healthy and infected roots of Rauwopfia serpentina was carried out by HPTLC analytical method. It was observed that there is decrease in reserpine content in infected roots.

 

Keywords:  Rauwolfia serpentina, Root, Reserpine, HPTLC, Macrophomina phaseolina.

_________________________________________________________________________

Editor: Dr.Arvind Chavhan

___________________________________________________________________________________ 

Cite this article as:

Dudhbhate MM  and  Kareppa BM (2018) Estimation of reserpine from healthy and diseased roots of Rauwopfia serpentina (L.) Benth. Ex. Kurz (Sarpagantdha) from different isolates by HPTLC analytical method, Int. J. of. Life Sciences, Volume 6(2): 688-699. 

____________________________________________________________________________


Conflicts of interest: The authors stated that no conflicts of interest.

____________________________________________________________________________

 

Copyright: © 2018 | Author(s), This is an open access article under the terms of the Creative Commons Attribution-Non-Commercial - No Derives License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

_________________________________________________________________________

 

Dhruv K Singh, Bhavana Srivastava, Archana Sahu, (2004). Spectrophotometric Determination of Rauwolfia Alkaloids: Estimation of Reserpine in Pharmaceuticals. Analytical Sciences; 20: 571‐573.


Indian Herbal Pharmacopoeia, Revised edition, Indian Drug Manufacturers Association: 345-354, (2002)


Indian Herbal Pharmacopoeia. Revised edition, Indian Drug Manufacturers Association, Mumbai 2002; 345‐354.


Kokate CK, Purohit AP, Gokhale SB, (2004). Pharmacognosy, 26th edition, Nirali Prakashan: 466- 470.


Kokate CK, Purohit AP, Gokhale SB, (2003). Pharmacognosy, Twenty Fourth Edition, Nirali Prakashan, Pune; 466‐470


Monograph number 9447. Merck Index. 12th Edition (Electronic version), 1999 Merck & Co., Inc., Whitehouse Station, NJ, USA.

Pulak K Mukherjee (2002). Quality Control of Herbal Drugs, 1st edition, Business Horizons: 120- 125.


Qureshi S A, Nawaz A, Udani SK, Anmi B (2009). Hypoglycaemic and Hypolipidemic Activities of Rauwolfia serpentina in Alloxan‐ Induced Diabetic Rats. International journal of Pharmacology; 1‐4.


Sameer Agarwal, Narayana BDA, Poonam Raghuvanshi, Srinivas KS, (1994). Quantitative Detection of β-Asarone in Acorus calamus using HPTLC. Indian Drugs, 32(6): 254 – 257.


Viel C, Galand N, Pothier J, Dollet J, OPLC and AMD (2002). Recent techniques of planar chromatography: Their interest for separation and characterization of extractive and synthetic compounds. Fitoterapia; 2‐14.


Wagner H, Bladt S, Zgainski EM, (1984). Plant Drug Analysis A Thin Layer Chromatography Atlas, Springer Verlag: 70-71.

 

 

 

IJLSCI EVENTS

ADVERTISEMENT

 
















    INTERNATIONAL JOURNAL OF

    LIFE SCIENCES

    Origin & Evolution

    Print ISSN : 2320-7817 

    Online ISSN:2320-964X

    UGC Approved Journal No. 48951


    EDITORIAL OFFICE

    46, Guruwandan, Jawahar Nagar, 

    VMV Road, Amravati- 444604

    Maharashtra, India.

    Tel  + 91- 9970559438  |   9420775527  

    Email: editor@ijlsci.in | editorijlsci@gmail.com