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Published yearly: 

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ISSN: 2320-964X (Online) 

ISSN: 2320-7817  (Print)



Dr. Santosh Pawar 




Int. Journal of Life Sciences, 2018; 6(2): 688-699      |                 Available online, April 28, 2018

Estimation of reserpine from healthy and diseased roots of Rauwopfia serpentina (L.) Benth. Ex. Kurz (Sarpagantdha) from different isolates by HPTLC analytical method


Dudhbhate MM1  and  Kareppa BM2


1Dept of Botany, ACS College, Gangakhed, MS, India

2Dept. of Botany, DSM College, Parbhani-431401.

*Corresponding author email:  |


Received : 16.01.2018     |    Revised : 31. 03.2018  |   Accepted : 23.04.2018   |   Published : 28.04.2018

Rauwolfia serpentina is an important medicinal herb used in Ayurveda and Alleopathy. Reserpine is an indole alkaloid present in Rauwolfia serpentina viz. reported to possess anti-hypertensive and tranquilizer property. Evaluation of herbal drug based on the amount of active constituent. Reserpine is present in all plant parts, but more in roots. Various factors are responsible for growth of plants and active constituent present in it. Roots are infected by fungi causing root rot disease that affect active constituent of root. Among the fungi, Macrophomina phaseolina causes severe root rot disease .In order to Changes in reserpine from healthy and infected roots of Rauwopfia serpentina, the healthy and infected roots of Sarpagandha was collected from four different places namely M. A., University, Parbhani designated HRS-1, IRS-1, Medicinal plant garden, M.P.K.V., Rahuri, designated HRS-2, IRS-2, Nagarjun medicinal plant garden P. D. K. V., Akola designated HRS-3, IRS-3 and S. G. M., Amravati University, Amravati designated HRS-4, IRS-4 during rainy season in the month of August 2009 was used for the analysis. In the present study, estimation of reserpine from healthy and infected roots of Rauwopfia serpentina was carried out by HPTLC analytical method. It was observed that there is decrease in reserpine content in infected roots.


Keywords:  Rauwolfia serpentina, Root, Reserpine, HPTLC, Macrophomina phaseolina.


Editor: Dr.Arvind Chavhan


Cite this article as:

Dudhbhate MM  and  Kareppa BM (2018) Estimation of reserpine from healthy and diseased roots of Rauwopfia serpentina (L.) Benth. Ex. Kurz (Sarpagantdha) from different isolates by HPTLC analytical method, Int. J. of. Life Sciences, Volume 6(2): 688-699. 


Conflicts of interest: The authors stated that no conflicts of interest.



Copyright: © 2018 | Author(s), This is an open access article under the terms of the Creative Commons Attribution-Non-Commercial - No Derives License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.



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