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Published yearly: 

4 Issues

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ISSN: 2320-964X (Online) 

ISSN: 2320-7817  (Print)

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Editor-in-Chief

Dr. Santosh Pawar 


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RESEARCH ARTICLE

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Int. Journal of Life Sciences, 2018; 6(4): 919-926      |                 Available online, December 22, 2018

Genetic polymorphisms of GSTM1 and GSTT1 and susceptibility to primary DNA damage in steel industry workers

 

U. Indira Priyadarshini1*, B. Vanitha1, Ch. Prashanth1, T. Sridevi5, P. Priyanka2, P. Pranay Krishna3 , SAA Latheef 4 and P.P Reddy1

 

1Department of Genetics, Bhagwan Mahavir Medical Research Centre,

2Department of Environmental Toxicology, Institute of Genetics and Hospital for Genetic Diseases,

3ACSR Government Hospital, Nellore Andhra Pradesh,

4Department of Genetics, Osmania University, Hyderabad, Telangana, India.

5Department of Biotechnology, Mahatma Gandhi National Institute of Research and Social Action

*Corresponding author email: indirapriyadarshini71@gmail.com

        

 

Received: 16.07.2018  |    Accepted: 29.11.2018     |    Published: 22.12.2018

The present study aims to investigate the possible association of detoxifying gene polymorphisms with DNA damage in steel industry workers. 150 steel industry workers and 146 control subjects with no history of occupational exposure to steel dust or any other chemicals were recruited for the study. DNA damage was evaluated using simple and reliable alkaline comet assay in peripheral blood lymphocytes of male steel industry workers. Analysis of GSTM1 and GSTT1 gene polymorphisms was done by multiplex PCR method. The results showed an increase in the frequency of DNA damage in peripheral blood lymphocytes of the steel industry workers compared to the control subjects. The statistical analysis of the results showed that significant DNA damage was observed in both genotypes of GSTM1 and GSTT1 but the frequency was found to be lower in steel industry workers with wild GSTM1 and GSTT1 genes than in those with null genotypes. The findings in this study presented evidence for the DNA damage highlighting an increased genotoxic risk as a result of exposure to dust in steel industry workers. The study also presented evidence for an association of both null genotypes of GSTM1 and GSTT1polymorphisms with DNA damage indicating the influence of GSTM1 and GSTT1 polymorphisms on this biomarker.

 

Keywords: DNA Damage, Occupational exposure to steel dust, Comet tail length, Gene Polymorphism

 

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Editor: Dr.Arvind Chavhan

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Cite this article as:

Indira Priyadarshini U, Vanitha B, Ch.Prashanth, Sridevi T,  Priyanka P, P Pranay Krishna, SAA Latheef and Reddy PP (2018) Genetic polymorphisms of GSTM1 and GSTT1 and susceptibility to primary DNA damage in steel industry workers, Int. J. of. Life Sciences, Volume 6(4): 919-926. 

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Acknowledgements

The authors wish to thank all the workers who participated in the study. We are also indebted to the management of the steel industries and Shri Motilal Jain, Chairman, Shri Mahendra Ranka, Vice-chairman, Shri Sushil Kapadia, Managing Trustee and Shri Ashok Kothari, Trustee Treasurer of our Research centre for their support and for providing necessary infrastructure facilities to carry out this project. 

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Conflicts of interest: The authors stated that no conflicts of interest.

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Copyright: © 2018 | Author(s), This is an open access article under the terms of the Creative Commons Attribution-Non-Commercial - No Derives License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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