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Published yearly: 

4 Issues


ISSN: 2320-964X (Online) 

ISSN: 2320-7817  (Print)



Dr. Santosh Pawar 




Int. Journal of Life Sciences, 2018; 6(4): 919-926      |                 Available online, December 22, 2018

Genetic polymorphisms of GSTM1 and GSTT1 and susceptibility to primary DNA damage in steel industry workers


U. Indira Priyadarshini1*, B. Vanitha1, Ch. Prashanth1, T. Sridevi5, P. Priyanka2, P. Pranay Krishna3 , SAA Latheef 4 and P.P Reddy1


1Department of Genetics, Bhagwan Mahavir Medical Research Centre,

2Department of Environmental Toxicology, Institute of Genetics and Hospital for Genetic Diseases,

3ACSR Government Hospital, Nellore Andhra Pradesh,

4Department of Genetics, Osmania University, Hyderabad, Telangana, India.

5Department of Biotechnology, Mahatma Gandhi National Institute of Research and Social Action

*Corresponding author email:



Received: 16.07.2018  |    Accepted: 29.11.2018     |    Published: 22.12.2018

The present study aims to investigate the possible association of detoxifying gene polymorphisms with DNA damage in steel industry workers. 150 steel industry workers and 146 control subjects with no history of occupational exposure to steel dust or any other chemicals were recruited for the study. DNA damage was evaluated using simple and reliable alkaline comet assay in peripheral blood lymphocytes of male steel industry workers. Analysis of GSTM1 and GSTT1 gene polymorphisms was done by multiplex PCR method. The results showed an increase in the frequency of DNA damage in peripheral blood lymphocytes of the steel industry workers compared to the control subjects. The statistical analysis of the results showed that significant DNA damage was observed in both genotypes of GSTM1 and GSTT1 but the frequency was found to be lower in steel industry workers with wild GSTM1 and GSTT1 genes than in those with null genotypes. The findings in this study presented evidence for the DNA damage highlighting an increased genotoxic risk as a result of exposure to dust in steel industry workers. The study also presented evidence for an association of both null genotypes of GSTM1 and GSTT1polymorphisms with DNA damage indicating the influence of GSTM1 and GSTT1 polymorphisms on this biomarker.


Keywords: DNA Damage, Occupational exposure to steel dust, Comet tail length, Gene Polymorphism



Editor: Dr.Arvind Chavhan


Cite this article as:

Indira Priyadarshini U, Vanitha B, Ch.Prashanth, Sridevi T,  Priyanka P, P Pranay Krishna, SAA Latheef and Reddy PP (2018) Genetic polymorphisms of GSTM1 and GSTT1 and susceptibility to primary DNA damage in steel industry workers, Int. J. of. Life Sciences, Volume 6(4): 919-926. 



The authors wish to thank all the workers who participated in the study. We are also indebted to the management of the steel industries and Shri Motilal Jain, Chairman, Shri Mahendra Ranka, Vice-chairman, Shri Sushil Kapadia, Managing Trustee and Shri Ashok Kothari, Trustee Treasurer of our Research centre for their support and for providing necessary infrastructure facilities to carry out this project. 


Conflicts of interest: The authors stated that no conflicts of interest.



Copyright: © 2018 | Author(s), This is an open access article under the terms of the Creative Commons Attribution-Non-Commercial - No Derives License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.


Baker  RS, Arlauskas A, Tandon RK, Crisp PT, and Ellis J(1986) Toxic and genotoxic action of electric arc welding fumes on cultured mammalian cells. J. Appl. Toxicol, 6: 357-362.

Bridges CC and Zalups RK(2005) Molecular and ionic mimicry and the transport of toxic metals. Toxicol Appl Pharmacol, 204: 274-308.

Brien TJ, Ceryak S, Patierno SR(2003) Complexities of chromium carcinogenesis: role of cellular response, repair and recovery mechanisms. Mutat Res, 533: 3-36.

Buschini A, De Palma G, Poli P, Martino A, Mozzoni P, Scotti E, Búzio L, Bergamaschi E, and Mutti A(2003) Genetic polymorphism of drug-metabolizing enzymes and styrene-induced DNA damage. Environ. Mol. Mutagen, 41: 243–52.

Chen, YY, Bye J, Yuang W, Chen J, Sun H, Wang H, Liang L, Guo X, Yang H, Tan Y, Su Q, Wei T, Wu(2006) Association of polymorphisms in AhR, CYP1A1, GSTM1, and GSTT1 genes with levels of DNA damage in peripheral blood lymphocytes among coke-oven workers. Cancer Epidemiol. Biomarkers Prev, 15: 1703–1707.

Cheng L(1997) Analysis of chromium (VI) mutagenic activity and specificity in yeast Saccharomyces system and Big Blue transgenic mouse system: molecular mechanisms of chromium (VI) mutagenesis. PhD Thesis, Cincinnati, OH:University of Cincinnati.

Collins AR, Dusinska M, Franklin M, Somorovska M, Petrovska H, Duthie S(1997) Comet assay in human biomonitoring studies: reliability, validation, and applications. Envir Mol Mutagen, 30:139–46.

Cornelia Richardson-Boedler (2002) Metal passivity as mechanism of metal carcinogenesis: Chromium, nickel, iron, copper, cobalt, platinum, molybdenum. Toxicological & Environmental Chemistry, 89:15-70. 

Cupo DY and Wetterhahn KE(1985) Modification of chromium (VI) - induced DNA damage by glutathione and cytochrome P-450 in chicken embryo hepatocytes. Proc Natl Acad Sci USA, 82:6755-6759.

Danadevi K, Roya Rozati B, Saleha Banu, Paramjit Grover (2004)  Genotoxic evaluation of welders occupationally exposed to chromium and nickel using the Comet and micronucleus assays, Mutagenesis, 19(1): 35–41.

De Flora S (2000) Threshold mechanisms and site specificity in chromium(VI) carcinogenesis. Carcinogenesis, 21: 533- 41.

De Flora S and Wetterhahn KE(1989) Mechanisms of chromium metabolism and genotoxicity. Life Chem Rep, 7:169-244.

Gibb HJ, Lees PS, Pinsky PF, Rooney BC(2000) Lung cancer among workers in chromium chemical production. Am J Ind Med, 38 (2):115-26.

Giri SK, Yadav A, Kumar A, Dev K, Gupta R, Aggarwal N, Gautam SK(2011) Association of  GSTM1 and GSTT1 polymorphisms on DNA damage in coal-tar workers. Science of the total environment, 409: 4465-69.

Hartwig A, Asmuss M, Ehleben I(2002) Interference by toxic metal ions with DNA repair processes and cell cycle control: molecular mechanisms. Environ Health Perspect, 110: 797-9.

Hayes RB (1997) The carcinogenicity of metals in humans. Cancer Causes Control8(3): 371- 85.

Hedenstedt A, Jenssen D, Lidestein BM (1977) Mutagenicity of fume particles from stainless steel welding. Scand J Work Environ Health, 3: 203-11.

Hengstler JG, Bolm-Audorff U, Faldum A (2003) Occupational exposure to heavy metals: DNA damage induction and DNA repair inhibition prove co-exposures to cadmium, cobalt and lead as more dangerous than hitherto expected. Carcinogenesis, 24: 63-73.

Iarmarcovai G, Bonassi S, Botta A, Baan RA, Orsiere T(2008) Genetic polymorphisms and micronucleus formation: a review of the literature. Mutat Res, 658: 215-33.

IndiraPriyadarshini U,Vanitha Baluka, Prashanth Ch,Reddy PP(2017) Epidemiological studies in workers Epidemiological Studies in Workers Exposed to Steel Dust internationalJ ournal of Advance Research, Ideas and Innovations in Technology, 3(6):888-894.                 


Karahalil B, Karakaya AE, Burgaz S (1999) The micronucleus assay in exfoliated buccal cells: application to occupational exposure to polycyclic aromatic hydrocarbons. Mutat Re, 442: 29-35.

Kasprzak KS, Jaruga P, Zastawny TH, North SL, Riggs CW, Olinski R, Dizdaroglu M(1997) Oxidative DNA base damage and its repair in kidneys and livers of nickel(II)-treated male F344 rats. Carcinogenesis,  18: 271–277.

Kassie F, Parzefall W, Knasmuller S (2000) Single cell gel electrophoresis assay: a new technique for human biomonitoring studies. Mutat Res, 463, 13-31.

Keshava N and Ong T (1999) Occupational exposure to genotoxic agents. Mutat Res, 155: 117-20.

Koshi K (1979) Effects of fume particles from stainless steel welding on sister chromatid exchanges and chromosome aberrations in cultured Chinese hamster cells. Ind. Health, 17: 39-49.

Langard S(1990) One hundred years of chromium and cancer: a review of epidemiological evidence and selected case reports. Am J Ind Med, 17: 189-215.

Lavender N A, Benford M L, VanCleave T T(2009) Examination of polymorphic glutathione S-transferase (GST) genes, tobacco smoking and prostate cancer risk among men of African descent: a case-control study. BMC Cancer, 9: 397. 

Leng S, Dai Y, Niu Y, Pan Z, Li X, Cheng J(2004) Effects of genetic polymorphisms of metabolic enzymes on cytokinesis-block micronucleus in peripheral blood lymphocyte among coke-oven workers. Cancer Epidemiol Biomark Prev, 13: 1631–9.

 Lide DR(1997) Handbook of chemistry and physics. 78th. Edition. CRC press, American chemical society.

Liu S, Dixon K(1996) Induction of mutagenic DNA damage by chromium (VI) and glutathione. Environ Mol Mutat, 28:71-79.

Maxild J, Andersen M, Kiel P (1978) Mutagenicity of fume particles from metal arc welding on stainless steel in the Salmonella/microsome test. Mutat Res, 56: 235-43.

Moretti M, DellOmo M, Villarini M, Pastorelli R, Muzi G, Airoldi L, Pasquini R(2007) Primary DNA damage and genetic polymorphisms for CYP1A1, EPHX and GSTM1 in workers at a graphite electrode manufacturing plant.BMC Public Health,7: 270.

Pool Zobel BL, Lotzmann N, Knoll M (1994) Detection of genotoxic effects in human gastric and nasal mucosa cells isolated from biopsy samples. Environ Mol Mutagen, 24: 23-45.

Quievryn G, Peterson E, Messer J, Zhitkovich A (2003) Genotoxicity and mutagenicity of chromium(VI)/ascorbate-generated DNA adducts in human and bacterial cells. Biochemistry, 42(4):1062-70.

Roberto Cappelletti, Marcello Ceppi, Justina Claudatus and Valerio Gennaro (2017) Health status of male steel workers at an electric arc furnace (EAF) in Trentino, Italy.Journal of Occupational Medicine and Toxicology, 11(7):1-10

Sabine and Martin (2009) Human Health Effects of Heavy Metals. Environmental Science and Technology Briefs for Citizens, 785(15): 532-6519.

Shi X, Chiu A, Chen CT, Halliwell B, Castranova V, Vallyathan V(1999) Reduction of chromium (VI) and its relationship to carcinogenesis. J Toxicol Environ Health, 2(1):87-104.

Singh NP, McCoy MT, Tice RR, Schneider EL(1988). A simple technique for quantitative of low levels of DNA damage in individual cells. Exp Cell Res, 175:184–91.

Sudha Sellappa1, Shibily Prathyumnan1, Kripa Subhadra Keyan1, Shyn Joseph1,B Sabari Guru Vasudevan1, K Sasikala(2010) Evaluation of DNA Damage Induction and Repair Inhibition in Welders Exposed to Hexavalent Chromium. Asian Pacific journal of cancer prevention,APJCP,(1):95-100.

Tchounwou PB, Yedjou CG, Patlolla AK, Sutton DJ(2012) Heavy metal toxicity and the environment. EXS,101:133-64. 

Tice RR(1995) The single cell gel/comet assay: a microgel electrophoretic technique for the detection of DNA damage and repair in individual cells. In Phillips DH, Venitt S (eds), Enivironmental Mutagenesis. Bios Scientific Publishers, Oxford, 315-339.

Tsapakos MJ, Hampton TH, Wetterhahn KE(1983) Chromium (VI) - induced DNA lesions and chromium distribution in rat kidney, liver, and lung. Cancer Res, 43:5662-5667.

Van Delft JHM, Roza L, Baan RA(1998) Biological effect markers for exposure to carcinogenic compound and their relevance for risk assessment. Crit Rev Toxicol, 28: 477- 510.

Wang Y, Wang SY, Jia L, Zhang L, Ba JC, Han D, Yu CP, Wu YH(2016) Nickel-Refining Fumes Induced DNA Damage and Apoptosis of NIH/3T3 Cells via Oxidative Stress. Int J Environ Res Public Health, 13(7).

Wise S S, Holmes A L, Ketterer M E, Hartsock W J, Fomchenko E, Katsifis S, Thompson W D and Wise J P(2004) Chromium is the proximate clastogenic species for lead-chromate induced clastogenicity in human bronchial cells. Mutation Research, 560: 79-89.

Wyllie AH, Kerr JFR, Currie AR(1980) Cell Death: The Significance of Apoptosis. Int Rev Cytol., 68:251-306.

Yang J, Hsieh Y, Wu C, Lee T(1992) Mutational specificity of chromium(VI) compounds in the hprt locus of Chinese hamster ovary-KI cells. Carcinogenesis, 13:2053-2057.

Zorawar Singh and Pooja Chadha(2015) Assessment of DNA damage as an index of genetic toxicity in welding microenvironments among iron-based industries. Toxicology and Industrial Health, 32(10):  1817-1824.








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