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Published yearly: 

4 Issues

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ISSN: 2320-964X (Online) 

ISSN: 2320-7817  (Print)

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Editor-in-Chief

Dr. Santosh Pawar 


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RESEARCH ARTICLE

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Int. Journal of Life Sciences, 2018; 6(4): 991-997      |                 Available online, December 31, 2018

Assessment of survival and ploidy condition of Zebra fish progeny produced through androgenesis using Disperms

 

D Sai Vinathi1, V Sridevi1, DC Reddy2, V Kalarani1*

 

1Department of Biotechnology, Sri Padmavati Mahila Visvavidyalayam, (Women’s University), TIRUPATI-517 502, A.P., INDIA

2Former UGC Emeritus Scientist, S.V. University, Tirupati

*Corresponding author email: kala.dandala@gmail.com

 

 

 

Received: 17.09.2018  |  Revised: 29.10.2018   |   Accepted: 23.12.2018   |   Published: 31.12.2018

Over exploitation, habitat modification, pollution load and introduction of exotic species affect fish genetic diversity directly or indirectly. Excessive mortality of fishes due to any of these factors lead to species reduction of population size or extinction. Hence conservation of aquatic genetic resources is essential to maintain ecological as well as socioeconomic equilibrium. Ex situ strategies like cloning of fish gametes and embryos are proven globally important. In conventional methods, production of androgenetic clones was done using X­ rays or γ­ rays followed by subjecting the eggs to thermal/ hydrostatic/ pressure shock for diploidization after monospermic activation. Due to two subsequent treatments of radiation and shock, the survival rates of putative androgenotes are presumed to be very low. The present study is carried out to assess the survival and ploidy condition of diploid androgenetic progeny of zebrafish, Danio frankei, produced through dispermic fertilization. Poor survival rates of hatchlings produced through fertilization of genome inactivated eggs with monosperms compared to the survival ability of hatchlings produced through fertilizing genome inactivated eggs with disperms was discussed. This study clearly showed that the use of combination of Poly Ethylene glycol (PEG) and Calcium chloride (CaCl2) is more effective than any single agent to form viable disperms that can induce fertilization of genome­ inactivated eggs similar to the effect shown by normal eggs.

 

Key words: Genome inactivated eggs, disperms, androgenetic clones, ploidy, 

 

 

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Editor: Dr.Arvind Chavhan

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Cite this article as:

D Sai Vinathi, V Sridevi, DC Reddy, V Kalarani (2018) Assessment of survival and ploidy condition of Zebra fish progeny produced through androgenesis using Disperms, Int. J. of. Life Sciences, Volume 6(4): 991-997. 

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Acknowledgements

Acknowledgments

This work was carried out as part of a Major Research Project (DST.Lr.No.SR/SO/AS-36 dated 17.05.2005) awarded to Prof. V. Kalarani by DST, New Delhi. The authors are grateful to Prof. T. J. Pandian, National Professor, Madurai Kamaraj University, Maduri for his guidance and support. Authors acknowledege DST – Curie, SPMVV, Tirupati for providing the lab facility.

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Conflicts of interest: The authors stated that no conflicts of interest.

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Copyright: © 2018 | Author(s), This is an open access article under the terms of the Creative Commons Attribution-Non-Commercial - No Derives License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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