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Int. Journal of Life Sciences, 2019; 7(1): 51-60      |                   Available online, March 28, 2019

Isolation, characterization of Endophytic Bacteria from Psidium guajava Plant and Screening of their Antibiotic Activities

Ajay Kumar Sahu1, Barsha Rani Kar2*, Santosh Sahu3, Pragnya Paramita Acharya4, and Priyadarsini SS Harichandan5

 

1Department of Microbiology, Bangalore University, Bangalore-560043, India |  2Department of Biotechnology, Utkal University, Bhubaneswar, Odisha  |   3Department of Biotechnology, Gardencity University, Bangalore

4Department of Biotechnology, Sambalpur University, Odisha, |  5Department of Biotechnology, Berhmpur University, Odisha, India

*Corresponding Author: bkar211099@gmail.com

 

Received : 21.12.2018   |   Accepted : 30.01.2019   |   Published : 28.03.2019

Endophytic bacteria defined as those bacteria that can be isolated from within the plant and that do not harm the plant visibly. These bacteria are thought to be virulent plant pathogens but have recently been discovered to have many beneficial effects on host plants, such as plant growth promotion, increased resistance against plant pathogen, parasites, etc. Besides gaining entry into the plants through natural openings or wounds, endophytic bacteria appear to actively penetrate plant tissues using hydrolytic enzymes like cellulose and pectins. Since these enzymes are also produced by pathogens, more information on their mechanism is needed to distinguish endophytic bacteria from plant pathogen. In general, an endophytic bacterium occurs in less number than pathogens, and at least some of them do not induce a hypersensitive response in the plant, indicating that they are not identified with the plant as pathogen. Six months old healthy plant of Psidium guajiava roots and stems were collected from the cultivated plant near Avulahalli, Bangalore, Karnataka. These plant samples were collected plant in sterile clean plastic bags brought to laboratory and used for further experimental purpose. These bacterial colonies were isolated from roots and one is isolated from the stem. The four bacterial colonies isolate were found to be may same species and they were shown common morphological and biochemical tests. An antibiotic sensitivity (or susceptibility) test is done to help choose antibiotic that will be most effective against the specific types of endophytic bacteria, root colony one and stem colony shown similar minimum inhibitory concentration (MIC).

 

Keywords: Endophytic bacteria, Psidium guajiava, Antimicrobial sensitivity, Biochemical tests, Stem, Root, MIC.

 

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Editor: Dr.Arvind Chavhan

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Cite this article as:

Ajay Kumar Sahu, Barsha Rani Kar, Santosh Sahu, Pragnya Paramita Acharya and Priyadarsini SS Harichandan (2019) Isolation, characterization of Endophytic Bacteria from Psidium guajava Plant and Screening of their Antibiotic Activities, Int. J. of. Life Sciences, Volume 7(1): 51-60. 

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Conflicts of interest: The authors stated that no conflicts of interest.

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Copyright: © 2019 | Author(s), This is an open access article under the terms of the Creative Commons Attribution-Non-Commercial - No Derives License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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