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IJLSCI

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ORIGINAL RESEARCH 

Volume 2 | Issue 2 |  June 2014 


Page Nos. 127- 133

Date of Online 30 June, 2014

 

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IJLSCI Vol. 5 (4) December 2017

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October 25, 2017

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Production of Bioactive Compound by Bacillus subtilis and its antagonistic activity against Sclerotium rolfsii


Gomashe Ashok V, Sheikh Neha AM and Gulhane Pranita A*

 

Department of Microbiology, S.S.E.S.A’s Science College, Congress Nagar, Nagpur-440012 (MS), India


*Corresponding Author email: pranita12@gmail.com

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ABSTRACT

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Bacillus subtilis has the potential to produce bioactive compound antagonistic against plant pathogenic fungi Sclerotium rolfsii. Bioassay test as well as agar well diffusion tests were performed which showed that Bacillus subtilis has produced the antifungal compound which was inhibitory against Sclerotium rolfsii. Chitin as a carbon source was found to be effective supplement for the effective production of bioactive compound. Another finding was that the bioactive compound produced by B. subtilis remained effective even after treated with different temperature and a wide pH range. The stability test showed that at 72 hours of incubation, at 370C and at pH 7, the maximum antagonistic activity (27mm and 26 mm respectively) was observed against S. rolfsii. The results obtained from the current study concluded that Bacillus subtilis and its bioactive compound can be used as a biological control agent for inhibition of phytopathogenic fungi Sclerotium rolfsii.


Keywords: Bacillus subtilis, Bioactive Compound, Chitin, Sclerotium rolfsii


 © 2014 |IJLSCI

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ARTICLE INFO

Received: 24 February, 2014  |  Revised: 30 May, 2014   |  Revised Recived:07 June, 2014

Accepted :14 June, 2014   |   Published : 30 June 2014


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Editor: Dr. Arvind Chavhan 

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Cite this article as:

Gomashe Ashok V, Sheikh Neha AM and Gulhane  Pranita (2014) A Production of Bioactive Compound by Bacillus subtilis and its antagonistic activity against Sclerotium rolfsii., Int. J. of Life Sciences, 2(2) : 127-133.

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Copyright: ©  2014 | Gomashe Ashok V, Sheikh Neha AM and Gulhane  Pranita A, This is an open access article under the terms of the Creative Commons Attribution NonCommercial- No Derivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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