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ORIGINAL RESEARCH

Volume 2 | Issue 1 |  March 2014 


Page Nos. 23- 29

Date of Online 30 March, 2014


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HPTLC Fingerprint Profile of Petroleum Ether Extracts from Wasteland Herb Cassia tora L.


Patil SD*, Wankhade SJ and Maknikar PP


Department of Microbiology, Shri Shivaji Science College, Amravati - 444603 (MS) India.


*Correspondence Author: Dr. Sahadeo D. Patil, Associate Professor and Head, Department of Microbiology, Shri Shivaji Science College, Amravati - 444603 Maharashtra, India. 

Email ID: sdpatil20957@yahoo.co.in

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ABSTRACT


Cassia tora L. (Family: Caesalpiniaceae) is a wild herb generally distributed in tropical countries. In India, it grows as a wasteland rainy season weed. Traditionally, Cassia tora L. is used for treating various ailments like leprosy, ringworm infection, ulcers and many more. The aim of the study is to develop a HPTLC fingerprint profile of petroleum ether extracts from aerial parts of Cassia tora. The HPTLC method was developed for the separation of phytoconstituents present in petroleum ether extracts using mobile phase of  n-Hexane: Ethyl acetate: formic acid: Acetic acid (60:40:2.5:2.5) and scanned under UV at 254 nm, 366nm and under white light. HPTLC fingerprinting of leaf extract displayed 10 constituents, seed (7 constituents) and flower (11 constituents). The HPTLC fingerprinting profile for petroleum ether extract may provide appropriate identification and quantification of non polar marker compounds and in the authentication of this plant.

 

Key Words: HPTLC, Cassia tora L., Medicinal plant, Standardization.

 

 © 2014 |IJLSCI

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ARTICLE INFO

Received: 28 December, 2013  

Finally accepted: 15 February, 2014

Date of publication (online): 30 March,  2014

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Editor: Dr. Arvind Chavhan 

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Citation: Patil SD, Wankhade SJ and Maknikar PP (2014) HPTLC Fingerprint Profile of Petroleum Ether Extracts from Wasteland Herb Cassia tora L., International Journal of Life Sciences, 2 (1): 23-29.

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Copyright: © Patil SDWankhade SJ and Maknikar PP, This is an open access article under the terms of the Creative Commons Attribution NonCommercial- No Derivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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