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Life Sciences

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RESEARCH ARTICLE

Volume 2 | Issue 4 |  December 2014 


Page Nos. 378- 381

Date of Online 30 December, 2014

 

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CALL FOR PAPERS

IJLSCI Vol. 5 (4) December 2017

Last Date for article submission

October 25, 2017

Email your Valuable Research to

 editorijlsci@gmail.com OR editor@ijlsci.in

  

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Statistical optimization of an amylase from Bacillus sp. by using response surface methodology

 

Kadam Omprakash A

 

Department of Microbiology, SSVP’S, Late Karmveer Dr. P.R. Ghogrey Science College, Dhule, MS, India.

Email: Omkadam2012@gmail.com  

 

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ABSTRACT

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There are four Bacillus sp. were tentatively identified by using culture dependent method. Among them one of the Bacillus sp. having potential to produce maximum extracellular amylase on skimmed milk agar plate.  The production of an extracellular amylase by Bacillus sp was statistically optimized by using response surface methodology (RSM). The maximum production of amylase from Bacillus sp. was achieved in presence of glucose and peptone as a carbon and nitrogen source respectively. The obtained ANOVA from central composite design showed a significant p value hence the designed model can be useful for to produce amylase by Bacillus sp.

 

Keywords: Bacillus sp., Amylase, RSM. 

 © 2014 |IJLSCI

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ARTICLE INFO

Received: 20 August, 2014   |   Revised : 25 September, 2014    |    Revised received: 10 October, 2014

Accepted: 01 December, 2014   |   Published :30 December, 2014

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Editor: Dr. Arvind Chavhan 

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Cite this article as:

Kadam Omprakash A (2014) Statistical optimization of an amylase from Bacillus sp. by using response surface methodology. Int.J. of Life Sciences, 2(4):378-381.

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Copyright: ©  2014 | Author(s), This is an open access article under the terms of the Creative Commons Attribution NonCommercial- No Derivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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