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Life Sciences

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RESEARCH ARTICLE

Volume 3 | Issue 1 |  March 2015 


Page Nos. 9-15

Date of Online 30 March, 2015

 

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CALL FOR PAPERS

IJLSCI Vol. 5 (3) September 2017

Last Date for article submission

July 25, 2017

Email your Valuable Research to

 editorijlsci@gmail.com OR editor@ijlsci.in

  

Streptomyces flavomacrosporus, A multi-metal tolerant potential bioremediation candidate isolated from paddy field irrigated with industrial effluents” 

Sunil KCR, Swati K, Bhavya G, Nandhini M, Veedashree M, Prakash HS, Kini KR, and Geetha N*

 


Eco-Biotech Laboratory, Department of Studies in Biotechnology, Manasagangotri,

University of Mysore, Mysuru, India – 570 006

                                                 

 *Corresponding author E-mail : geetha@appbot.uni-mysore.ac.in

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ABSTRACT

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Prime drive of the research work carried out was to explore heavy metal-tolerant bacteria from rhizospheric soil samples collected from contaminated agricultural lands of southern India. Soil samples of Mysore agricultural lands of Karnataka state, India, irrigated with industrial effluents from several decades, were brought to the laboratory in sterile polythene bags and were screened by plating serially diluted samples on to Minimal Glucose Yeast extract agar media amended with 0.3mM mercury. Morphologically differing cultures were tested for their tolerance to mercury by growing them in the liquid minimal media amended with 0.3, 0.4, 0.5 and 0.6mM concentrations of mercuric chloride and 0.5, 1.0 and 1.5mM of lead nitrate at optimal physiological conditions. Further the isolates were identified using 16s r-DNA PCR amplification. Blast analysis of the sequence results revealed for the first time the tolerance potential of Streptomyces flavomacrosporus, Bacillus methylotrophicus, Achromobacter xylosoxidans, Bacillus tequilensis, Bacillus pumilus, and Bacillus subtilis and their chances of participation in bioremediation of mercury. Among these isolates the actinomycetes S. flavomacrosporus, showed tolerance to multi metals.

 

Keywords– Heavy Metals, Minimal Glucose Yeast Extract (MGY), Industrial effluents, Agricultural lands, Metal-tolerant bacteria.

 © 2015 |IJLSCI

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ARTICLE INFO

Received: 04 January, 2015     |     Revised : 20 February, 2015

Accepted: 28 February, 2015  |      Published : 30 March, 2015

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Editor: Dr. Arvind Chavhan 

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Cite this article as:

Sunil KCR, Swati K, Bhavya G, Nandhini M, Veedashree M, Prakash HS, Kini KR, and and Geetha N (2015) “Streptomyces flavomacrosporus, A multi-metal tolerant potential bioremediation candidate isolated from paddy field irrigated with industrial effluents” Int. J. of Life Sciences, 3(1): 9- 15.

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Copyright: ©  2015 | Author(s), This is an open access article under the terms of the Creative Commons Attribution NonCommercial- No Derivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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